Product: DNAJB4 Antibody
Catalog: DF3594
Description: Rabbit polyclonal antibody to DNAJB4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 38 KD; 38kD(Calculated).
Uniprot: Q9UDY4
RRID: AB_2835966

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(80%), Horse(90%), Sheep(80%), Rabbit(100%), Dog(80%)
Clonality:
Polyclonal
Specificity:
DNAJB4 Antibody detects endogenous levels of total DNAJB4.
RRID:
AB_2835966
Cite Format: Affinity Biosciences Cat# DF3594, RRID:AB_2835966.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DjB4; DnaJ (Hsp40) homolog subfamily B member 4; DnaJ homolog subfamily B member 4; DnaJ like heat shock protein 40; DNAJB 4; DNAJB4; DNAJW; DNJB4_HUMAN; Heat shock 40 kDa protein 1 homolog; Heat shock protein 40 homolog; HLJ 1; HLJ1; HSP40 homolog; Human liver DnaJ-like protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UDY4 DNJB4_HUMAN:

Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver.

Sequence:
MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
90
Pig
80
Bovine
80
Sheep
80
Dog
80
Chicken
70
Xenopus
63
Zebrafish
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UDY4 As Substrate

Site PTM Type Enzyme
K13 Ubiquitination
Y24 Phosphorylation
K30 Ubiquitination
K37 Ubiquitination
S38 Phosphorylation
K46 Ubiquitination
K59 Ubiquitination
K60 Ubiquitination
K73 Ubiquitination
T79 Phosphorylation
T85 Phosphorylation
S122 Phosphorylation
S133 Phosphorylation
S138 Phosphorylation
S148 Phosphorylation
S167 Phosphorylation
Y172 Phosphorylation
C175 S-Nitrosylation
K177 Acetylation
K213 Ubiquitination
T223 Phosphorylation
K236 Ubiquitination
S248 Phosphorylation
Y252 Phosphorylation
S280 Phosphorylation
K286 Ubiquitination
Y296 Phosphorylation
K302 Ubiquitination
K332 Ubiquitination

Research Backgrounds

Function:

Probable chaperone. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Cytoplasmic according to PubMed:18837411 and membrane-associated according to PubMed:16542645.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver.

Subunit Structure:

Homodimer. The C-terminal section interacts with the C-terminal tail of OPRM1. Interacts also with SDIM1.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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