Product: BARD1 Antibody
Catalog: DF3605
Description: Rabbit polyclonal antibody to BARD1
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 79 KD; 87kD(Calculated).
Uniprot: Q99728
RRID: AB_2835977

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

BARD1 Antibody detects endogenous levels of total BARD1.
Cite Format: Affinity Biosciences Cat# DF3605, RRID:AB_2835977.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


BARD-1; Bard1; BARD1_HUMAN; BRCA1 associated RING domain 1; BRCA1 associated RING domain gene 1; BRCA1 associated RING domain protein 1; BRCA1-associated RING domain protein 1;



PTMs - Q99728 As Substrate

Site PTM Type Enzyme
K46 Sumoylation
K110 Ubiquitination
K124 Acetylation
K124 Ubiquitination
K130 Ubiquitination
K139 Ubiquitination
K144 Sumoylation
S148 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
K152 Acetylation
K160 Ubiquitination
K170 Sumoylation
K170 Ubiquitination
S184 Phosphorylation
S186 Phosphorylation
S192 Phosphorylation
K217 Sumoylation
K217 Ubiquitination
K224 Ubiquitination
S244 Phosphorylation
S251 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S288 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
T299 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
K305 Sumoylation
Y307 Phosphorylation
S313 Phosphorylation
S330 Phosphorylation
S342 Phosphorylation
S344 Phosphorylation
S364 Phosphorylation
S367 Phosphorylation
K371 Acetylation
S391 Phosphorylation
T394 Phosphorylation
T398 Phosphorylation
S402 Phosphorylation
S410 Phosphorylation
S412 Phosphorylation
K423 Sumoylation
K423 Ubiquitination
T430 Phosphorylation
S443 Phosphorylation
Y446 Phosphorylation
K457 Ubiquitination
K484 Ubiquitination
S496 Phosphorylation
K548 Sumoylation
K548 Ubiquitination
S586 Phosphorylation
K596 Ubiquitination
K650 Ubiquitination
K688 Ubiquitination
S704 Phosphorylation
T714 Phosphorylation
T719 Phosphorylation
T734 Phosphorylation

Research Backgrounds


E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.


Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.

Subcellular Location:

Note: During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homo- and heterodimer. Heterodimer (RING-type zinc finger) with BRCA1. Heterodimer (via ANK repeats and BRCT domains) with CSTF1/CSTF-50. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Interacts with UBXN1.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

Restrictive clause


Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.