Product: CYB5 Antibody
Catalog: DF3609
Description: Rabbit polyclonal antibody to CYB5
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Chicken
Mol.Wt.: 15 KD; 15kD(Calculated).
Uniprot: P00167
RRID: AB_2835981

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Chicken(88%)
Clonality:
Polyclonal
Specificity:
CYB5 Antibody detects endogenous levels of total CYB5.
RRID:
AB_2835981
Cite Format: Affinity Biosciences Cat# DF3609, RRID:AB_2835981.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CYB 5; CYB 5A; CYB5; CYB5_HUMAN; Cyb5a; Cytochrome b 5; Cytochrome b5 (microsomal); Cytochrome b5; Cytochrome b5 type A (microsomal); Cytochrome b5 type A; MCB 5; MCB5; Microsomal cytochrome b5; Microsomal cytochrome b5 type A; Type 1 cyt b5;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
88
Dog
75
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00167 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
K10 Ubiquitination
K19 Acetylation
K19 Ubiquitination
S25 Phosphorylation
Y35 Phosphorylation
K39 Acetylation
K39 Ubiquitination
R52 Methylation
T60 Phosphorylation
T70 Phosphorylation

Research Backgrounds

Function:

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein>Cytoplasmic side. Microsome membrane>Single-pass membrane protein>Cytoplasmic side.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the cytochrome b5 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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