Product: NXF1 Antibody
Catalog: AF0375
Description: Rabbit polyclonal antibody to NXF1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 70kDa; 70kD(Calculated).
Uniprot: Q9UBU9
RRID: AB_2833536

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 100ul $280 In stock
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Product Info

WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(80%), Bovine(80%), Sheep(80%), Dog(80%), Chicken(100%), Xenopus(80%)
NXF1 Antibody detects endogenous levels of total NXF1.
Cite Format: Affinity Biosciences Cat# AF0375, RRID:AB_2833536.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


DKFZp667O0311; DmNXF1; MEX67; MEX67, yeast, homolog of; Mex67p; mRNA export factor TAP; Mvb1; Nuclear RNA export factor 1; Nuclear RNA export factor 1 homolog (S. cerevisiae); nxf 1; NXF1; NXF1_HUMAN; Protein small bristles; Sbr; TAP; Tip associating protein; Tip-associated protein; Tip-associating protein;



Expressed ubiquitously.

NXF1 Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5. Belongs to the NXF family. Heterodimer with NXT1. Interacts (via NTF2 domain) with NXT1. The formation of NXF1/NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Interacts with NXT2, E1B-AP5, RAE1, THOC4 and with several nucleoporins. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, RENT1, RENT2, RENT3A, RENT3B, THOC4 and/or REFBP2. Found in a mRNA complex with RENT3A and RENT3B. Interacts with FYTTD1/UIF, EIF4A3, NUPL2 and THOC5. Interacts with Saimiriine herpesvirus 2 TIP protein



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBU9 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K6 Ubiquitination
S9 Phosphorylation
Y33 Phosphorylation
S62 Phosphorylation
Y72 Phosphorylation
Y75 Phosphorylation
T76 Phosphorylation
T77 Phosphorylation
R82 Methylation
T95 Phosphorylation
R105 Methylation
K117 Ubiquitination
K121 Ubiquitination
C143 S-Nitrosylation
T148 Phosphorylation
K173 Ubiquitination
K178 Ubiquitination
S188 Phosphorylation
K205 Ubiquitination
K218 Ubiquitination
S223 Phosphorylation
K230 Methylation
K230 Ubiquitination
S251 Phosphorylation
K289 Ubiquitination
K294 Ubiquitination
S299 Phosphorylation
K304 Sumoylation
K304 Ubiquitination
K347 Ubiquitination
K374 Ubiquitination
K437 Ubiquitination
T554 Phosphorylation
S556 Phosphorylation
S557 Phosphorylation
S558 Phosphorylation
T562 Phosphorylation
S564 Phosphorylation
K607 Ubiquitination
K609 Ubiquitination

Research Backgrounds


Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.

Subcellular Location:

Nucleus. Nucleus>Nucleoplasm. Nucleus speckle. Nucleus>Nuclear pore complex. Nucleus envelope. Cytoplasm. Cytoplasm>Stress granule.
Note: Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. The association with the TREX complex seems to occur in regions surrounding nuclear speckles known as perispeckles (PubMed:23826332). Nucleus; nuclear rim (PubMed:25662211).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously.

Subunit Structure:

Heterodimer (via NTF2 domain) with NXT1. The formation of NXF1-NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Forms a complex with RANBP2/NUP358, NXT1 and RANGAP1. Associates with the exon junction complex (EJC) and with the transcription/export (TREX) complex. Found in a mRNA complex with UPF3A and UPF3B. Found in a post-splicing complex with RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1. Interacts (via N-terminus) with DHX9 (via N-terminus); this interaction is direct and negatively regulates NXF1-mediated nuclear export of constitutive transport element (CTE)-containing cellular mRNAs. Interacts with ALYREF/THOC4. Interacts with FYTTD1/UIF. Interacts with EIF4A3. Interacts with NUP42. Interacts with THOC5. Interacts with CHTOP. Interacts with FRG1 (via N-terminus). Interacts with LUZP4. Interacts with FMR1; the interaction occurs in a mRNA-dependent and polyribosomes-independent manner in the nucleus. Interacts with CPSF6 (via N-terminus); this interaction is direct. Interacts with RBM15. Interacts with RBM15B. Interacts with MCM3AP isoform GANP; this interaction is not mediated by RNA. Interacts with DDX3X (via C-terminus); this interaction may be partly involved in DDX3X nuclear export and in NXF1 localization to stress granules. Interacts with PABPC1/PABP1.

(Microbial infection) Interacts with Saimiriine herpesvirus 2 TIP protein.

(Microbial infection) Interacts with human herpes virus 1 (HHV-1) ICP27 protein; this interaction allows efficient export of HHV-1 early and late transcripts.


The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.

The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.

The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus. It inhibits RNA binding activity through an intramolecular interaction with the N-terminal RNA binding domain (RBD); the inhibition is removed by an association with the TREX complex, specifically involving ALYREF/THOC4 and THOC5.

The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.

The leucine-rich repeats are essential for the export of mRNA from the nucleus.

The RNA-binding domain is a non-canonical RNP-type domain.

Belongs to the NXF family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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