ORC1L Antibody | Affinity Biosciences

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ORC1L Antibody - Western blot analysis of extracts from HepG2 cells, using ORC1L antibody.

Product:	ORC1L Antibody
Catalog:	DF3637
Description:	Rabbit polyclonal antibody to ORC1L
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	100,120 KD; 97kD(Calculated).
Uniprot:	Q13415
RRID:	AB_2836009

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(91%)

Clonality:

Polyclonal

Specificity:

ORC1L Antibody detects endogenous levels of total ORC1L.

RRID:

AB_2836009
Cite Format: Affinity Biosciences Cat# DF3637, RRID:AB_2836009.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HSORC1; MmORC1; orc1; ORC1_HUMAN; ORC1L; Origin Recognition Complex 1; Origin recognition complex subunit 1 (yeast homolog) like; Origin recognition complex subunit 1; Origin recognition complex subunit 1 homolog; Origin recognition complex subunit 1 like (S. cerevisia; Origin recognition complex subunit 1 like; Origin recognition complex subunit 1 S. cerevisiae homolog like; Origin recognition complex, subunit 1 like (yeast); PARC1; Replication control protein 1;

Immunogens

Immunogen:

Uniprot:

Q13415(ORC1_HUMAN) >>Visit HPA database.

Gene(ID):

ORC1(4998)

Sequence:

Q13415 ORC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAHYPTRLKTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDDENPYVAKLLELFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQEIFWYDYPACDSNINAETIIGLVRVIPLAPKDVVPTNLKNEKTLFVKLSWNEKKFRPLSSELFAELNKPQESAAKCQKPVRAKSKSAESPSWTPAEHVAKRIESRHSASKSRQTPTHPLTPRARKRLELGNLGNPQMSQQTSCASLDSPGRIKRKVAFSEITSPSKRSQPDKLQTLSPALKAPEKTRETGLSYTEDDKKASPEHRIILRTRIAASKTIDIREERTLTPISGGQRSSVVPSVILKPENIKKRDAKEAKAQNEATSTPHRIRRKSSVLTMNRIRQQLRFLGNSKSDQEEKEILPAAEISDSSSDEEEASTPPLPRRAPRTVSRNLRSSLKSSLHTLTKVPKKSLKPRTPRCAAPQIRSRSLAAQEPASVLEEARLRLHVSAVPESLPCREQEFQDIYNFVESKLLDHTGGCMYISGVPGTGKTATVHEVIRCLQQAAQANDVPPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAELLAKQFCTRGSPQETTVLLVDELDLLWTHKQDIMYNLFDWPTHKEARLVVLAIANTMDLPERIMMNRVSSRLGLTRMCFQPYTYSQLQQILRSRLKHLKAFEDDAIQLVARKVAALSGDARRCLDICRRATEICEFSQQKPDSPGLVTIAHSMEAVDEMFSSSYITAIKNSSVLEQSFLRAILAEFRRSGLEEATFQQIYSQHVALCRMEGLPYPTMSETMAVCSHLGSCRLLLVEPSRNDLLLRVRLNVSQDDVLYALKDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Horse

Dog

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13415 As Substrate

Q13415

Site	PTM Type	Enzyme	Source
K25	Sumoylation		Uniprot
K25	Ubiquitination		Uniprot
K81	Ubiquitination		Uniprot
K99	Ubiquitination		Uniprot
K149	Ubiquitination		Uniprot
K152	Ubiquitination		Uniprot
K163	Ubiquitination		Uniprot
K164	Ubiquitination		Uniprot
K178	Ubiquitination		Uniprot
S182	Phosphorylation		Uniprot
S194	Phosphorylation		Uniprot
K195	Ubiquitination		Uniprot
S196	Phosphorylation		Uniprot
S199	Phosphorylation		Uniprot
S201	Phosphorylation		Uniprot
T203	Phosphorylation		Uniprot
K210	Ubiquitination		Uniprot
S217	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot
T224	Phosphorylation		Uniprot
T226	Phosphorylation		Uniprot
T230	Phosphorylation		Uniprot
S255	Phosphorylation		Uniprot
S258	Phosphorylation	P06493 (CDK1) , P24941 (CDK2)	Uniprot
S269	Phosphorylation		Uniprot
S273	Phosphorylation	P06493 (CDK1) , P24941 (CDK2)	Uniprot
S275	Phosphorylation		Uniprot
T285	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
S311	Phosphorylation		Uniprot
K326	Acetylation		Uniprot
T337	Phosphorylation		Uniprot
S340	Phosphorylation		Uniprot
S350	Phosphorylation		Uniprot
K364	Acetylation		Uniprot
T373	Phosphorylation		Uniprot
S374	Phosphorylation		Uniprot
T375	Phosphorylation	P24941 (CDK2) , P06493 (CDK1)	Uniprot
S383	Phosphorylation		Uniprot
S384	Phosphorylation		Uniprot
T387	Phosphorylation		Uniprot
S417	Phosphorylation		Uniprot
S419	Phosphorylation		Uniprot
S420	Phosphorylation		Uniprot
S421	Phosphorylation		Uniprot
S427	Phosphorylation		Uniprot
K448	Methylation		Uniprot
K448	Ubiquitination		Uniprot
S449	Phosphorylation		Uniprot
S450	Phosphorylation		Uniprot
T453	Phosphorylation		Uniprot
T455	Phosphorylation		Uniprot
K456	Ubiquitination		Uniprot
S461	Phosphorylation		Uniprot
S476	Phosphorylation		Uniprot
S478	Phosphorylation		Uniprot
K540	Ubiquitination		Uniprot
K586	Ubiquitination		Uniprot
K591	Ubiquitination		Uniprot
K603	Ubiquitination		Uniprot
S610	Phosphorylation		Uniprot
K698	Ubiquitination		Uniprot
K711	Ubiquitination		Uniprot
S850	Phosphorylation		Uniprot
K859	Ubiquitination		Uniprot

Research Backgrounds

Q13415_ORC1_HUMAN

Function:

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

PTMs:

Phosphorylated during mitosis.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated.

Family&Domains:

The BAH domain mediates binding to dimethylated histone H4 'Lys-20' (H4K20me2), which is enriched at replication origins.

Belongs to the ORC1 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

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ORC1L Antibody - #DF3637