PSMD11 Antibody - #DF3643

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Product: PSMD11 Antibody
Catalog: DF3643
Description: Rabbit polyclonal antibody to PSMD11
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 42 KD; 47kD(Calculated).
Uniprot: O00231
RRID: AB_2836015

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
PSMD11 Antibody detects endogenous levels of total PSMD11.
RRID:
AB_2836015
Cite Format: Affinity Biosciences Cat# DF3643, RRID:AB_2836015.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit 9; 26S proteasome regulatory subunit p44.5; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; MGC3844; p44.5; protease 26S, subunit, 9; proteasome (prosome, macropain) 26S subunit, non-ATPase, 11; proteasome 26S subunit, non-ATPase, 11; PSD11_HUMAN; PSMD 11; PSMD11; Rpn6; S9;

Immunogens

Immunogen:
Uniprot:

O00231(PSD11_HUMAN) >>Visit HPA database.

Gene(ID):
PSMD11(5717)
Expression:
O00231 PSD11_HUMAN:

Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.

Sequence:
MAAAAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00231 As Substrate

Site PTM Type Enzyme
A2 Acetylation
R11 Methylation
S14 Phosphorylation P17612 (PRKACA)
R20 Methylation
S23 Phosphorylation
S29 Phosphorylation
K32 Ubiquitination
K46 Ubiquitination
S49 Phosphorylation
S55 Phosphorylation
K59 Ubiquitination
T60 Phosphorylation
K71 Ubiquitination
Y72 Phosphorylation
S79 Phosphorylation
S81 Phosphorylation
K82 Ubiquitination
Y137 Phosphorylation
K141 Acetylation
K141 Ubiquitination
S151 Phosphorylation
K175 Ubiquitination
S181 Phosphorylation
K185 Ubiquitination
Y201 Phosphorylation
C202 S-Nitrosylation
K205 Ubiquitination
T209 Phosphorylation
S214 Phosphorylation
K223 Ubiquitination
K246 Ubiquitination
K252 Ubiquitination
S272 Phosphorylation
K274 Ubiquitination
T284 Phosphorylation
K288 Ubiquitination
C289 S-Nitrosylation
K295 Ubiquitination
S298 Phosphorylation
K304 Methylation
K304 Ubiquitination
S320 Phosphorylation
K325 Ubiquitination
K358 Ubiquitination
K364 Ubiquitination
K372 Ubiquitination
T396 Phosphorylation
Y397 Phosphorylation
S413 Phosphorylation
Y415 Phosphorylation
K417 Acetylation
K417 Ubiquitination

Research Backgrounds

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

PTMs:

Phosphorylated by AMPK.

Subcellular Location:

Nucleus. Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD11, a base containing 6 ATPases and few additional components.

Family&Domains:

Belongs to the proteasome subunit S9 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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