ABCF1 Antibody - #AF0385

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Product: ABCF1 Antibody
Catalog: AF0385
Description: Rabbit polyclonal antibody to ABCF1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 95kDa; 96kD(Calculated).
Uniprot: Q8NE71
RRID: AB_2833541

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
ABCF1 Antibody detects endogenous levels of total ABCF1.
RRID:
AB_2833541
Cite Format: Affinity Biosciences Cat# AF0385, RRID:AB_2833541.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABC27; ABC50; ABCF1; ABCF1_HUMAN; ATP binding cassette 50 (TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; ATP binding cassette, sub family F (GCN20), member 1; ATP-binding cassette 50; ATP-binding cassette sub-family F member 1; AU041969; D17Wsu166e; EST123147; GCN20; TNF alpha stimulated ABC protein; TNF-alpha-stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667;

Immunogens

Immunogen:
Uniprot:

Q8NE71(ABCF1_HUMAN) >>Visit HPA database.

Gene(ID):
ABCF1(23)
Expression:
Q8NE71 ABCF1_HUMAN:

Ubiquitous.

Description:
ABCF1 May function in mRNA translation. Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. Induced by TNF (in cultured synoviocytes). Interacts with eukaryotic initiation factor 2 (eIF2). Associates with the ribosomes. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Sequence:
MPKAPKQQPPEPEWIGDGESTSPSDKVVKKGKKDKKIKKTFFEELAVEDKQAGEEEKVLKEKEQQQQQQQQQQKKKRDTRKGRRKKDVDDDGEEKELMERLKKLSVPTSDEEDEVPAPKPRGGKKTKGGNVFAALIQDQSEEEEEEEKHPPKPAKPEKNRINKAVSEEQQPALKGKKGKEEKSKGKAKPQNKFAALDNEEEDKEEEIIKEKEPPKQGKEKAKKAEQGSEEEGEGEEEEEEGGESKADDPYAHLSKKEKKKLKKQMEYERQVASLKAANAAENDFSVSQAEMSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIANRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLKLLEEERRLQGQLEQGDDTAAERLEKVYEELRATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDAQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEALTRKQQKCRRKNQDEESQEAPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYQGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTHGEMRKNHRLKIGFFNQQYAEQLRMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFEDYKREVLEALGEVMVSRPRE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Pig
91
Xenopus
91
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NE71 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
S20 Phosphorylation
T21 Phosphorylation
S22 Phosphorylation
S24 Phosphorylation
K26 Ubiquitination
K39 Ubiquitination
K50 Ubiquitination
S105 Phosphorylation
T108 Phosphorylation
S109 Phosphorylation P68400 (CSNK2A1)
S140 Phosphorylation P68400 (CSNK2A1)
K163 Acetylation
K163 Methylation
S166 Phosphorylation
K174 Methylation
K174 Ubiquitination
K176 Methylation
K209 Ubiquitination
S228 Phosphorylation
S244 Phosphorylation
Y250 Phosphorylation
S254 Phosphorylation
K255 Ubiquitination
K263 Ubiquitination
Y267 Phosphorylation
S273 Phosphorylation
K275 Methylation
K275 Ubiquitination
S287 Phosphorylation
K305 Ubiquitination
K308 Ubiquitination
Y332 Phosphorylation
T343 Phosphorylation
T344 Phosphorylation
K347 Ubiquitination
K389 Ubiquitination
T407 Phosphorylation
K414 Ubiquitination
Y416 Phosphorylation
T422 Phosphorylation
K430 Ubiquitination
K451 Ubiquitination
K537 Methylation
K549 Ubiquitination
Y551 Phosphorylation
K573 Ubiquitination
K576 Ubiquitination
T580 Phosphorylation
K582 Ubiquitination
K589 Ubiquitination
S595 Phosphorylation
K603 Ubiquitination
S621 Phosphorylation
S652 Phosphorylation
S665 Phosphorylation
T666 Phosphorylation
T672 Phosphorylation
T676 Phosphorylation
T678 Phosphorylation
K689 Ubiquitination
Y710 Phosphorylation
K748 Ubiquitination

Research Backgrounds

Function:

Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.

PTMs:

Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.

Subcellular Location:

Cytoplasm. Nucleus>Nucleoplasm. Nucleus envelope.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Isoform 2 interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Isoform 2 associates (via both ABC transporter domains) with the ribosomes.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.

Restrictive clause

 

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