MRPL47 Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562 cells using MRPL47 antibody.

Product:	MRPL47 Antibody
Catalog:	DF3673
Description:	Rabbit polyclonal antibody to MRPL47
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	30 KD; 29kD(Calculated).
Uniprot:	Q9HD33
RRID:	AB_2836045

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

MRPL47 Antibody detects endogenous levels of total MRPL47.

RRID:

AB_2836045
Cite Format: Affinity Biosciences Cat# DF3673, RRID:AB_2836045.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

39S ribosomal protein L47 mitochondrial; 39S ribosomal protein L47, mitochondrial; CGI 204; L47mt; MGC45403; Mitochondrial ribosomal protein L47; MRP L47; MRP-L47; MRPL 47; MRPL47; Nasopharyngeal carcinoma metastasis related 1; Nasopharyngeal carcinoma metastasis related protein 1; Nasopharyngeal carcinoma metastasis-related protein 1; NCM1; RM47_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human MRPL47, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9HD33(RM47_HUMAN) >>Visit HPA database.

Gene(ID):

MRPL47(57129)

Sequence:

Q9HD33 RM47_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAGLALLCRRVSSALKSSRSLITPQVPACTGFFLSLLPKSTPNVTSFHQYRLLHTTLSRKGLEEFFDDPKNWGQEKVKSGAAWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLDKVVDSMDALDKVVQEREDALRLLQTGQERARPGAWRRDIFGRIIWHKFKQWVIPWHLNKRYNRKRFFALPYVDHFLRLEREKRARIKARKENLERKKAKILLKKFPHLAEAQKSSLV

PTMs - Q9HD33 As Substrate

Q9HD33

Site	PTM Type	Source
T43	Phosphorylation	Uniprot
T47	Phosphorylation	Uniprot
K62	Ubiquitination	Uniprot
K72	Ubiquitination	Uniprot
K78	Ubiquitination	Uniprot
K80	Ubiquitination	Uniprot
C87	S-Nitrosylation	Uniprot
S138	Phosphorylation	Uniprot
K144	Acetylation	Uniprot
K237	Ubiquitination	Uniprot

Research Backgrounds

Q9HD33_RM47_HUMAN

Subcellular Location:

Mitochondrion.

Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.

Family&Domains:

Belongs to the universal ribosomal protein uL29 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MRPL47 Antibody - #DF3673