Product: AZI1 Antibody
Catalog: DF3692
Description: Rabbit polyclonal antibody to AZI1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Sheep, Dog
Mol.Wt.: 130 KD; 122kD(Calculated).
Uniprot: Q9UPN4
RRID: AB_2836056

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
AZI1 Antibody detects endogenous levels of total AZI1.
Cite Format: Affinity Biosciences Cat# DF3692, RRID:AB_2836056.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


5 azacytidine induced 1; 5-azacytidine-induced protein 1; AZ1; Azi; Azi1; AZI1_HUMAN; Centrosomal protein 131 kDa; Centrosomal protein of 131 kDa; Cep131; KIAA1118; OTTMUSP00000004498; Pre-acrosome localization protein 1; RP23 37J21.1;



A synthesized peptide derived from human AZI1, corresponding to a region within C-terminal amino acids.




Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPN4 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S21 Phosphorylation
S35 Phosphorylation
K40 Ubiquitination
S45 Phosphorylation
S47 Phosphorylation P49137 (MAPKAPK2)
S52 Phosphorylation
K57 Ubiquitination
S76 Phosphorylation
S78 Phosphorylation P49137 (MAPKAPK2)
T79 Phosphorylation
T80 Phosphorylation
S87 Phosphorylation
S89 Phosphorylation
T93 Phosphorylation
T96 Phosphorylation
S105 Phosphorylation
S107 Phosphorylation
K110 Ubiquitination
S114 Phosphorylation
S116 Phosphorylation
S120 Phosphorylation
K122 Ubiquitination
T137 Phosphorylation
S144 Phosphorylation
S145 Phosphorylation
S146 Phosphorylation
S150 Phosphorylation
K157 Acetylation
K157 Ubiquitination
K175 Ubiquitination
K200 Methylation
K200 Ubiquitination
S208 Phosphorylation
K214 Methylation
K214 Ubiquitination
S221 Phosphorylation
K228 Ubiquitination
K231 Ubiquitination
T244 Phosphorylation
S247 Phosphorylation
K254 Ubiquitination
T257 Phosphorylation
K300 Ubiquitination
S308 Phosphorylation
K320 Ubiquitination
K346 Ubiquitination
K353 Ubiquitination
S381 Phosphorylation
T383 Phosphorylation
K393 Ubiquitination
T413 Phosphorylation
S416 Phosphorylation
S417 Phosphorylation
S447 Phosphorylation
S450 Phosphorylation
S453 Phosphorylation
S499 Phosphorylation
K510 Ubiquitination
T522 Phosphorylation
S525 Phosphorylation
S531 Phosphorylation
S534 Phosphorylation
S542 Phosphorylation
K577 Ubiquitination
T601 Phosphorylation
S613 Phosphorylation
K647 Ubiquitination
K656 Ubiquitination
K678 Ubiquitination
K681 Ubiquitination
K689 Ubiquitination
S697 Phosphorylation
K699 Ubiquitination
K704 Ubiquitination
T707 Phosphorylation
K717 Ubiquitination
K730 Methylation
K730 Ubiquitination
S731 Phosphorylation
S745 Phosphorylation
S798 Phosphorylation
T857 Phosphorylation
K859 Ubiquitination
K876 Ubiquitination
K932 Ubiquitination
K955 Ubiquitination
S990 Phosphorylation
S991 Phosphorylation
S994 Phosphorylation
R1007 Methylation
K1019 Ubiquitination
T1024 Phosphorylation
T1079 Phosphorylation

Research Backgrounds


Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation. In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner. Acts also as a negative regulator of BBSome ciliary trafficking. Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail (By similarity). Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability in non-ciliogenic cells. Involved in centriole duplication (By similarity). Required for CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2.


Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation. Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation.

MAPKAPK2-dependent phosphorylation at Ser-47 and Ser-78 occurs in response to cellular stress such as exposure to ultraviolet irradiation and promotes binding to 14-3-3 proteins which leads to cytoplasmic sequestration of CEP131 and blocks formation of new centriolar satellites.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasmic vesicle>Secretory vesicle>Acrosome.
Note: Colocalized with pericentriolar material protein PCM1 at centriolar satellites. During spermiogenesis, becomes enriched with nephrocystin NPHP1 at the transition zone, a structure at the base of the ciliary axoneme important for regulating traffic into the ciliary compartment. Traffics towards and away from the centrosome/basal body and the transition zone of the ciliary axoneme in a microtubule-dependent manner. Localized at the Golgi-derived acrosome and the centrosome-containing head-tail coupling apparatus (HTCA) (By similarity). Ubiquitinated form is sequestered and colocalized with BBS4, CEP290, PCM1 and PCNT at centriolar satellites in proliferating cells. Colocalized with the pericentriolar material protein PCM1 at centrosome. Traffics towards and away from centriolar satellites and centrosome in a microtubule- and dynein-dependent manner in interphase cells. Displaced from centriolar satellites but still remains associated with the centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAPK signaling (PubMed:26616734).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Self-associates. Associates with the centriolar satellite BBSome protein complex. Interacts with BBS4; the interaction limits BBS4 availability for association with the BBSome complex, and hence negatively regulates ciliary localization of the BBSome complex. Interacts with MIB1. Interacts with PCM1; the interaction increases in response to ultraviolet light (UV) radiation. Associates with microtubules; association with microtubules is reduced in response to cellular stress, such as UV stimulation, in a process that requires p38 MAP kinase signaling. Interacts with CEP290, DCTN1, PCNT, PCM1 and CEP152. Interacts with 14-3-3 proteins following UV-induced phosphorylation by MAPKAPK2; this inhibits formation of novel centriolar satellites. Interacts with SDCCAG8.


Belongs to the CEP131 family.

Restrictive clause


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