ABHD12 Antibody | Affinity Biosciences

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Western blot analysis of extracts from VERO cells(H2O2 treatment), using ABHD12 Antibody.

Product:	ABHD12 Antibody
Catalog:	DF3717
Description:	Rabbit polyclonal antibody to ABHD12
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	45 KD; 45kD(Calculated).
Uniprot:	Q8N2K0
RRID:	AB_2836081

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ABHD12 Antibody detects endogenous levels of total ABHD12.

RRID:

AB_2836081
Cite Format: Affinity Biosciences Cat# DF3717, RRID:AB_2836081.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2-arachidonoylglycerol hydrolase; 6330583M11Rik; ABD12_HUMAN; ABHD12; ABHD12A; Abhydrolase domain containing protein 12; Abhydrolase domain-containing protein 12; AI431047; AW547313; BEM46L2; C20orf22; Monoacylglycerol lipase ABHD12; RP23-241M12.2;

Immunogens

Immunogen:

A synthesized peptide derived from human ABHD12, corresponding to a region within C-terminal amino acids.

Uniprot:

Q8N2K0(ABD12_HUMAN) >>Visit HPA database.

Gene(ID):

ABHD12(26090)

Sequence:

Q8N2K0 ABD12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRKRTEPVALEHERCAAAGSSSSGSAAAALDADCRLKQNLRLTGPAAAEPRCAADAGMKRALGRRKGVWLRLRKILFCVLGLYIAIPFLIKLCPGIQAKLIFLNFVRVPYFIDLKKPQDQGLNHTCNYYLQPEEDVTIGVWHTVPAVWWKNAQGKDQMWYEDALASSHPIILYLHGNAGTRGGDHRVELYKVLSSLGYHVVTFDYRGWGDSVGTPSERGMTYDALHVFDWIKARSGDNPVYIWGHSLGTGVATNLVRRLCERETPPDALILESPFTNIREEAKSHPFSVIYRYFPGFDWFFLDPITSSGIKFANDENVKHISCPLLILHAEDDPVVPFQLGRKLYSIAAPARSFRDFKVQFVPFHSDLGYRHKYIYKSPELPRILREFLGKSEPEHQH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Chicken

Rabbit

Horse

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N2K0 As Substrate

Q8N2K0

Site	PTM Type	Source
T5	Phosphorylation	Uniprot
T43	Phosphorylation	Uniprot
Y110	Phosphorylation	Uniprot
S195	Phosphorylation	Uniprot
K232	Ubiquitination	Uniprot
S235	Phosphorylation	Uniprot
Y241	Phosphorylation	Uniprot
S246	Phosphorylation	Uniprot
T249	Phosphorylation	Uniprot
T253	Phosphorylation	Uniprot
S273	Phosphorylation	Uniprot
K283	Ubiquitination	Uniprot
K319	Ubiquitination	Uniprot
Y345	Phosphorylation	Uniprot
K358	Ubiquitination	Uniprot

Research Backgrounds

Q8N2K0_ABD12_HUMAN

Function:

Lysophosphatidylserine (LPS) lipase that mediates the hydrolysis of lysophosphatidylserine, a class of signaling lipids that regulates immunological and neurological processes. Represents a major lysophosphatidylserine lipase in the brain, thereby playing a key role in the central nervous system (By similarity). Also able to hydrolyze oxidized phosphatidylserine; oxidized phosphatidylserine is produced in response to severe inflammatory stress and constitutes a proapoptotic 'eat me' signal. Also has monoacylglycerol (MAG) lipase activity: hydrolyzes 2-arachidonoylglycerol (2-AG), thereby acting as a regulator of endocannabinoid signaling pathways. Has a strong preference for very-long-chain lipid substrates; substrate specificity is likely due to improved catalysis and not improved substrate binding.

PTMs:

Glycosylated; glycosylation is required for optimal activity.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Family&Domains:

Belongs to the serine esterase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ABHD12 Antibody - #DF3717