ABHD7 Antibody - #DF3721
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Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(83%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ABHD7 Antibody detects endogenous levels of total ABHD7.
RRID:
AB_2836085
Cite Format: Affinity Biosciences Cat# DF3721, RRID:AB_2836085.
Cite Format: Affinity Biosciences Cat# DF3721, RRID:AB_2836085.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ABHD7; Abhydrolase domain containing 7; Abhydrolase domain-containing protein 7; Ephx4; EPHX4_HUMAN; EPHXRP; Epoxide hydrolase 4; Epoxide hydrolase-related protein;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q8IUS5 EPHX4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARLRDCLPRLMLTLRSLLFWSLVYCYCGLCASIHLLKLLWSLGKGPAQTFRRPAREHPPACLSDPSLGTHCYVRIKDSGLRFHYVAAGERGKPLMLLLHGFPEFWYSWRYQLREFKSEYRVVALDLRGYGETDAPIHRQNYKLDCLITDIKDILDSLGYSKCVLIGHDWGGMIAWLIAICYPEMVMKLIVINFPHPNVFTEYILRHPAQLLKSSYYYFFQIPWFPEFMFSINDFKVLKHLFTSHSTGIGRKGCQLTTEDLEAYIYVFSQPGALSGPINHYRNIFSCLPLKHHMVTTPTLLLWGENDAFMEVEMAEVTKIYVKNYFRLTILSEASHWLQQDQPDIVNKLIWTFLKEETRKKD
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Sheep
92
Bovine
83
Zebrafish
70
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IUS5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y85 | Phosphorylation | Uniprot | |
| Y130 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| T149 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot |
Research Backgrounds
Subcellular Location:
Membrane>Single-pass type II membrane protein.
Family&Domains:
Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
References
1). ABHD7-mediated depalmitoylation of lamin A promotes myoblast differentiation. Cell reports, 2024
(PubMed: 38308845)
[IF=8.8]
Application: WB Species: Human Sample: HEK293T cells
Figure 3ABHD7 is the depalmitoylase of lamin A (A) Exogenous ABHD5, -7, -17A, -17C, and -18 interact with lamin A. Myc-tagged lamin A was co-expressed with FLAG-tagged ABHDs, PPTs, or APTs in HEK293T cells, followed by immunoprecipitation for western blotting. (B) ABHD7 interacts with endogenous lamin A. Endogenous lamin A was detected by western blotting in HEK293T cells expressing FLAG-tagged ABHDs. (C) Overexpression of ABHD7 reduces palmitoylation of lamin A in HEK293T cells. The palmitoylation of lamin A was detected by ABE assay in HEK293T cells expressing FLAG-tagged lamin A and Myc-tagged ABHD7. “Ratio,” biotin/FLAG. (D) Knocking down ABHD7 increases exogenous palmitoylation of lamin A. The palmitoylation of FLAG-tagged lamin A was detected in ABHD7-knockdown HEK293T cells through ABE assay. “Ratio,” biotin/FLAG. (E) Endogenous palmitoylation of lamin A is increased in C2C12 cells with Abhd7 knockdown. The palmitoylation of lamin A was determined through ABE assay. “Ratio,” biotin/lamin A. (F) Immunofluorescence staining shows co-localization of Abhd7 (green) and lamin A/C (red) at the nucleus region. Scale bar: 50 μm. Data in (A)–(F) are representative of three biologically independent experiments.
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