APOA4 Antibody | Affinity Biosciences

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APOA4 Antibody - Western blot analysis of APOA4 using CACO-2 whole cell lysates.

Product:	APOA4 Antibody
Catalog:	DF3782
Description:	Rabbit polyclonal antibody to APOA4
Application:	WB
Reactivity:	Human
Mol.Wt.:	45 KD; 45kD(Calculated).
Uniprot:	P06727
RRID:	AB_2836139

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

APOA4 Antibody detects endogenous levels of total APOA4.

RRID:

AB_2836139
Cite Format: Affinity Biosciences Cat# DF3782, RRID:AB_2836139.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Apo AIV; APOA 4; ApoA IV; APOA4; Apolipoprotein A IV; Apolipoprotein A4; MGC142154; MGC142156;

Immunogens

Immunogen:

Uniprot:

P06727(APOA4_HUMAN) >>Visit HPA database.

Expression:

P06727 APOA4_HUMAN:

Synthesized primarily in the intestine and secreted in plasma.

Sequence:

P06727 APOA4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFLKAVVLTLALVAVAGARAEVSADQVATVMWDYFSQLSNNAKEAVEHLQKSELTQQLNALFQDKLGEVNTYAGDLQKKLVPFATELHERLAKDSEKLKEEIGKELEELRARLLPHANEVSQKIGDNLRELQQRLEPYADQLRTQVNTQAEQLRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGRLTPYADEFKVKIDQTVEELRRSLAPYAQDTQEKLNHQLEGLTFQMKKNAEELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQVEEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDKVNSFFSTFKEKESQDKTLSLPELEQQQEQQQEQQQEQVQMLAPLES

PTMs - P06727 As Substrate

P06727

Site	PTM Type	Source
K65	Ubiquitination	Uniprot
S174	Phosphorylation	Uniprot
T202	Phosphorylation	Uniprot
S257	Phosphorylation	Uniprot
S259	Phosphorylation	Uniprot
Y310	Phosphorylation	Uniprot

Research Backgrounds

P06727_APOA4_HUMAN

Function:

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

PTMs:

Phosphorylation sites are present in the extracellular medium.

Subcellular Location:

Secreted.

Tissue Specificity:

Synthesized primarily in the intestine and secreted in plasma.

Subunit Structure:

Homodimer.

Family&Domains:

Nine of the thirteen 22-amino acid tandem repeats (each 22-mer is actually a tandem array of two, A and B, related 11-mers) occurring in this sequence are predicted to be highly alpha-helical, and many of these helices are amphipathic. They may therefore serve as lipid-binding domains with lecithin:cholesterol acyltransferase (LCAT) activating abilities.

Belongs to the apolipoprotein A1/A4/E family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

APOA4 Antibody - #DF3782