APOF Antibody - #DF3784

(2)  
Product: APOF Antibody
Catalog: DF3784
Description: Rabbit polyclonal antibody to APOF
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 38 KD; 35kD(Calculated).
Uniprot: Q13790
RRID: AB_2836141

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
APOF Antibody detects endogenous levels of total APOF.
RRID:
AB_2836141
Cite Format: Affinity Biosciences Cat# DF3784, RRID:AB_2836141.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Apo-F; Apof; APOF_HUMAN; Apolipoprotein F; DKFZp781G18150; Lipid transfer inhibitor protein; LTIP; MGC22520;

Immunogens

Immunogen:
Uniprot:

Q13790(APOF_HUMAN) >>Visit HPA database.

Gene(ID):
APOF(319)
Expression:
Q13790 APOF_HUMAN:

Expressed by the liver and secreted in plasma.

Sequence:
MTGLCGYSAPDMRGLRLIMIPVELLLCYLLLHPVDATSYGKQTNVLMHFPLSLESQTPSSDPLSCQFLHPKSLPGFSHMAPLPKFLVSLALRNALEEAGCQADVWALQLQLYRQGGVNATQVLIQHLRGLQKGRSTERNVSVEALASALQLLAREQQSTGRVGRSLPTEDCENEKEQAVHNVVQLLPGVGTFYNLGTALYYATQNCLGKARERGRDGAIDLGYDLLMTMAGMSGGPMGLAISAALKPALRSGVQQLIQYYQDQKDANISQPETTKEGLRAISDVSDLEETTTLASFISEVVSSAPYWGWAIIKSYDLDPGAGSLEI

PTMs - Q13790 As Substrate

Site PTM Type Enzyme
T274 O-Glycosylation
S323 Phosphorylation

Research Backgrounds

Function:

Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.

PTMs:

O-glycosylated with core 1 or possibly core 8 glycans.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by the liver and secreted in plasma.

Family&Domains:

Belongs to the apolipoprotein F family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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