ARFIP1 Antibody - #DF3790

(3)  
Product: ARFIP1 Antibody
Catalog: DF3790
Description: Rabbit polyclonal antibody to ARFIP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 40 KD; 42kD(Calculated).
Uniprot: P53367
RRID: AB_2836147

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ARFIP1 Antibody detects endogenous levels of total ARFIP1.
RRID:
AB_2836147
Cite Format: Affinity Biosciences Cat# DF3790, RRID:AB_2836147.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosylation factor interacting protein 1; ADP-ribosylation factor-interacting protein 1; Arfaptin; Arfaptin-1; ARFIP 1; Arfip1; ARFP1_HUMAN; HSU52521; MGC117369;

Immunogens

Immunogen:
Uniprot:

P53367(ARFP1_HUMAN) >>Visit HPA database.

Gene(ID):
ARFIP1(27236)
Expression:
P53367 ARFP1_HUMAN:

Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.

Sequence:
MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGSPAPPLPSVMSPSRVAASRLAQQGSDLIVPAGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLKTPGVDAPSWLEEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
83
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53367 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
S9 Phosphorylation
S24 Phosphorylation
S28 Phosphorylation
K34 Ubiquitination
S36 Phosphorylation
S39 Phosphorylation
S44 Phosphorylation
T46 Phosphorylation
S69 Phosphorylation
S76 Phosphorylation
S79 Phosphorylation
S93 Phosphorylation
K107 Ubiquitination
S108 Phosphorylation
K118 Ubiquitination
K124 Ubiquitination
S132 Phosphorylation Q15139 (PRKD1)
T135 Phosphorylation
Y136 Phosphorylation
K146 Ubiquitination
K222 Ubiquitination
K258 Ubiquitination
T272 Phosphorylation
K289 Ubiquitination
K301 Ubiquitination
Y304 Phosphorylation
K316 Ubiquitination
K322 Ubiquitination
K353 Ubiquitination
K360 Ubiquitination
T361 Phosphorylation
S368 Phosphorylation

Research Backgrounds

Function:

Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanisitically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.

PTMs:

Phosphorylated by PRKD1; phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery.

Subcellular Location:

Golgi apparatus. Golgi apparatus>trans-Golgi network membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.

Subunit Structure:

Forms homodimers or heterodimers with ARFIP2. Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3. Interacts with ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6. Interacts with ARL1.

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