ATXN2 Antibody - #DF3799

(2)  
Product: ATXN2 Antibody
Catalog: DF3799
Description: Rabbit polyclonal antibody to ATXN2
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 140 KD; 140kD(Calculated).
Uniprot: Q99700
RRID: AB_2836156

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(82%), Bovine(80%), Horse(91%), Sheep(90%), Rabbit(91%), Dog(91%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
ATXN2 Antibody detects endogenous levels of total ATXN2.
RRID:
AB_2836156
Cite Format: Affinity Biosciences Cat# DF3799, RRID:AB_2836156.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ASL13; Ataxin 2; Ataxin-2; ATX2_HUMAN; Atxn2; Olivopontocerebellar ataxia 2, autosomal dominant; SCA2; Spinocerebellar ataxia type 2 protein; TNRC13; Trinucleotide repeat containing 13; Trinucleotide repeat containing gene 13 protein; Trinucleotide repeat-containing gene 13 protein;

Immunogens

Immunogen:
Uniprot:

Q99700(ATX2_HUMAN) >>Visit HPA database.

Gene(ID):
ATXN2(6311)
Expression:
Q99700 ATX2_HUMAN:

Expressed in the brain, heart, liver, skeletal muscle, pancreas and placenta. Isoform 1 is predominant in the brain and spinal cord. Isoform 4 is more abundant in the cerebellum. In the brain, broadly expressed in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.

Sequence:
MRSAAAAPRSPAVATESRRFAAARWPGWRSLQRPARRSGRGGGGAAPGPYPSAAPPPPGPGPPPSRQSSPPSASDCFGSNGNGGGAFRPGSRRLLGLGGPPRPFVVLLLPLASPGAPPAAPTRASPLGARASPPRSGVSLARPAPGCPRPACEPVYGPLTMSLKPQQQQQQQQQQQQQQQQQQQQQQQPPPAAANVRKPGGSGLLASPAAAPSPSSSSVSSSSATAPSSVVAATSGGGRPGLGRGRNSNKGLPQSTISFDGIYANMRMVHILTSVVGSKCEVQVKNGGIYEGVFKTYSPKCDLVLDAAHEKSTESSSGPKREEIMESILFKCSDFVVVQFKDMDSSYAKRDAFTDSAISAKVNGEHKEKDLEPWDAGELTANEELEALENDVSNGWDPNDMFRYNEENYGVVSTYDSSLSSYTVPLERDNSEEFLKREARANQLAEEIESSAQYKARVALENDDRSEEEKYTAVQRNSSEREGHSINTRENKYIPPGQRNREVISWGSGRQNSPRMGQPGSGSMPSRSTSHTSDFNPNSGSDQRVVNGGVPWPSPCPSPSSRPPSRYQSGPNSLPPRAATPTRPPSRPPSRPSRPPSHPSAHGSPAPVSTMPKRMSSEGPPRMSPKAQRHPRNHRVSAGRGSISSGLEFVSHNPPSEAATPPVARTSPSGGTWSSVVSGVPRLSPKTHRPRSPRQNSIGNTPSGPVLASPQAGIIPTEAVAMPIPAASPTPASPASNRAVTPSSEAKDSRLQDQRQNSPAGNKENIKPNETSPSFSKAENKGISPVVSEHRKQIDDLKKFKNDFRLQPSSTSESMDQLLNKNREGEKSRDLIKDKIEPSAKDSFIENSSSNCTSGSSKPNSPSISPSILSNTEHKRGPEVTSQGVQTSSPACKQEKDDKEEKKDAAEQVRKSTLNPNAKEFNPRSFSQPKPSTTPTSPRPQAQPSPSMVGHQQPTPVYTQPVCFAPNMMYPVPVSPGVQPLYPIPMTPMPVNQAKTYRAVPNMPQQRQDQHHQSAMMHPASAAGPPIAATPPAYSTQYVAYSPQQFPNQPLVQHVPHYQSQHPHVYSPVIQGNARMMAPPTHAQPGLVSSSATQYGAHEQTHAMYACPKLPYNKETSPSFYFAISTGSLAQQYAHPNATLHPHTPHPQPSATPTGQQQSQHGGSHPAPSPVQHHQHQAAQALHLASPQQQSAIYHAGLAPTPPSMTPASNTQSPQNSFPAAQQTVFTIHPSHVQPAYTNPPHMAHVPQAHVQSGMVPSHPTAHAPMMLMTTQPPGGPQAALAQSALQPIPVSTTAHFPYMTHPSVQAHHQQQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Dog
91
Chicken
91
Rabbit
91
Sheep
90
Pig
82
Bovine
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99700 As Substrate

Site PTM Type Enzyme
R2 Methylation
S3 Phosphorylation
R9 Methylation
R40 Methylation
R244 Methylation
R246 Methylation
S248 Phosphorylation
Y263 Phosphorylation
Y290 Phosphorylation
Y297 Phosphorylation
S298 Phosphorylation
K300 Ubiquitination
C301 S-Nitrosylation
K311 Ubiquitination
S327 Phosphorylation
Y347 Phosphorylation
K349 Ubiquitination
S356 Phosphorylation
K361 Ubiquitination
S393 Phosphorylation
S431 Phosphorylation
K436 Ubiquitination
Y454 Phosphorylation
K455 Ubiquitination
R465 Methylation
S466 Phosphorylation
K470 Ubiquitination
Y471 Phosphorylation
T472 Phosphorylation
S478 Phosphorylation
S505 Phosphorylation
S508 Phosphorylation
R510 Methylation
S513 Phosphorylation
R515 Methylation
S521 Phosphorylation
S523 Phosphorylation
S526 Phosphorylation
S528 Phosphorylation
S530 Phosphorylation
S554 Phosphorylation
S558 Phosphorylation
S560 Phosphorylation
S565 Phosphorylation
S569 Phosphorylation
S573 Phosphorylation
T580 Phosphorylation
S600 Phosphorylation
S616 Phosphorylation
S617 Phosphorylation
R622 Methylation
S624 Phosphorylation
R635 Methylation
S637 Phosphorylation
R640 Methylation
S642 Phosphorylation
T660 Phosphorylation
R665 Methylation
T666 Phosphorylation
S667 Phosphorylation
S669 Phosphorylation
T672 Phosphorylation
S678 Phosphorylation
S684 Phosphorylation
S692 Phosphorylation
S697 Phosphorylation
S728 Phosphorylation
T730 Phosphorylation
S733 Phosphorylation
S736 Phosphorylation
T741 Phosphorylation
S743 Phosphorylation
S744 Phosphorylation
S749 Phosphorylation
S758 Phosphorylation
T771 Phosphorylation
S772 Phosphorylation
S774 Phosphorylation
S776 Phosphorylation
K781 Ubiquitination
S784 Phosphorylation
S788 Phosphorylation
T811 Phosphorylation
S814 Phosphorylation
K821 Ubiquitination
S828 Phosphorylation
S839 Phosphorylation
S848 Phosphorylation
S849 Phosphorylation
S850 Phosphorylation
S854 Phosphorylation
S856 Phosphorylation
S857 Phosphorylation
S861 Phosphorylation
S863 Phosphorylation
S865 Phosphorylation
S867 Phosphorylation
S870 Phosphorylation
T872 Phosphorylation
T887 Phosphorylation
S888 Phosphorylation
S889 Phosphorylation
K911 Ubiquitination
T913 Phosphorylation
T934 Phosphorylation
R998 Methylation
Y1105 Phosphorylation

Research Backgrounds

Function:

Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the brain, heart, liver, skeletal muscle, pancreas and placenta. Isoform 1 is predominant in the brain and spinal cord. Isoform 4 is more abundant in the cerebellum. In the brain, broadly expressed in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.

Subunit Structure:

Monomer (By similarity). Can also form homodimers (By similarity). Interacts with TARDBP; the interaction is RNA-dependent. Interacts with RBFOX1. Interacts with polyribosomes. Interacts with SH3GL2 and SH3GL3. Interacts with SH3KBP1 and CBL (By similarity). Interacts with EGFR. Interacts with ATXN2L.

Family&Domains:

Belongs to the ataxin-2 family.

Restrictive clause

 

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