ATXN2L Antibody - #DF3800

(2)  
Product: ATXN2L Antibody
Catalog: DF3800
Description: Rabbit polyclonal antibody to ATXN2L
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 113 KD; 113kD(Calculated).
Uniprot: Q8WWM7
RRID: AB_2836157

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ATXN2L Antibody detects endogenous levels of total ATXN2L.
RRID:
AB_2836157
Cite Format: Affinity Biosciences Cat# DF3800, RRID:AB_2836157.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A2D; A2LG; A2LP; A2RP; Ataxin 2 domain protein; Ataxin 2 like; Ataxin 2 like protein; Ataxin 2 related protein; Ataxin-2 domain protein; Ataxin-2 like; Ataxin-2-like protein; Ataxin-2-related protein; ATX2L_HUMAN; ATXN2L; ATXN2L ataxin 2 like;

Immunogens

Immunogen:
Uniprot:

Q8WWM7(ATX2L_HUMAN) >>Visit HPA database.

Gene(ID):
ATXN2L(11273)
Expression:
Q8WWM7 ATX2L_HUMAN:

Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.

Sequence:
MLKPQPLQQPSQPQQPPPTQQAVARRPPGGTSPPNGGLPGPLATSAAPPGPPAAASPCLGPVAAAGSGLRRGAEGILAPQPPPPQQHQERPGAAAIGSARGQSTGKGPPQSPVFEGVYNNSRMLHFLTAVVGSTCDVKVKNGTTYEGIFKTLSSKFELAVDAVHRKASEPAGGPRREDIVDTMVFKPSDVMLVHFRNVDFNYATKDKFTDSAIAMNSKVNGEHKEKVLQRWEGGDSNSDDYDLESDMSNGWDPNEMFKFNEENYGVKTTYDSSLSSYTVPLEKDNSEEFRQRELRAAQLAREIESSPQYRLRIAMENDDGRTEEEKHSAVQRQGSGRESPSLASREGKYIPLPQRVREGPRGGVRCSSSRGGRPGLSSLPPRGPHHLDNSSPGPGSEARGINGGPSRMSPKAQRPLRGAKTLSSPSNRPSGETSVPPPPAVGRMYPPRSPKSAAPAPISASCPEPPIGSAVPTSSASIPVTSSVSDPGVGSISPASPKISLAPTDVKELSTKEPGRTLEPQELARIAGKVPGLQNEQKRFQLEELRKFGAQFKLQPSSSPENSLDPFPPRILKEEPKGKEKEVDGLLTSEPMGSPVSSKTESVSDKEDKPPLAPSGGTEGPEQPPPPCPSQTGSPPVGLIKGEDKDEGPVAEQVKKSTLNPNAKEFNPTKPLLSVNKSTSTPTSPGPRTHSTPSIPVLTAGQSGLYSPQYISYIPQIHMGPAVQAPQMYPYPVSNSVPGQQGKYRGAKGSLPPQRSDQHQPASAPPMMQAAAAAGPPLVAATPYSSYIPYNPQQFPGQPAMMQPMAHYPSQPVFAPMLQSNPRMLTSGSHPQAIVSSSTPQYPSAEQPTPQALYATVHQSYPHHATQLHAHQPQPATTPTGSQPQSQHAAPSPVQHQAGQAPHLGSGQPQQNLYHPGALTGTPPSLPPGPSAQSPQSSFPQPAAVYAIHHQQLPHGFTNMAHVTQAHVQTGITAAPPPHPGAPHPPQVMLLHPPQSHGGPPQGAVPQSGVPALSASTPSPYPYIGHPQGEQPGQAPGFPGGADDRIREFSLAGGIWHGRAEGLQVGQDARVLGGE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WWM7 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T31 Phosphorylation
S32 Phosphorylation
S45 Phosphorylation
S56 Phosphorylation
S67 Phosphorylation
R71 Methylation
S98 Phosphorylation
S103 Phosphorylation
K106 Ubiquitination
S111 Phosphorylation
Y118 Phosphorylation
T128 Phosphorylation
K138 Ubiquitination
K140 Ubiquitination
Y145 Phosphorylation
K150 Ubiquitination
K155 Ubiquitination
K166 Ubiquitination
S168 Phosphorylation
Y202 Phosphorylation
T204 Phosphorylation
K205 Ubiquitination
K207 Ubiquitination
S211 Phosphorylation
K218 Ubiquitination
S236 Phosphorylation
S238 Phosphorylation
Y241 Phosphorylation
Y264 Phosphorylation
K267 Ubiquitination
Y270 Phosphorylation
S272 Phosphorylation
S275 Phosphorylation
S276 Phosphorylation
Y277 Phosphorylation
T278 Phosphorylation
K283 Ubiquitination
S286 Phosphorylation
S305 Phosphorylation
S306 Phosphorylation
Y309 Phosphorylation
T322 Phosphorylation
S335 Phosphorylation
S339 Phosphorylation
S341 Phosphorylation
S344 Phosphorylation
K348 Acetylation
K348 Ubiquitination
Y349 Phosphorylation
R361 Methylation
R365 Methylation
R370 Methylation
R373 Methylation
S377 Phosphorylation
R382 Methylation
S390 Phosphorylation
S391 Phosphorylation
S396 Phosphorylation
R399 Methylation
S406 Phosphorylation
S409 Phosphorylation
R414 Methylation
R417 Methylation
T421 Phosphorylation
S423 Phosphorylation
S424 Phosphorylation
S426 Phosphorylation
R428 Methylation
S430 Phosphorylation
Y445 Phosphorylation
S449 Phosphorylation
S491 Phosphorylation
S493 Phosphorylation
S496 Phosphorylation
S500 Phosphorylation
K507 Ubiquitination
S510 Phosphorylation
T511 Phosphorylation
K529 Ubiquitination
K538 Ubiquitination
R539 Methylation
R546 Methylation
K547 Ubiquitination
K553 Methylation
K553 Ubiquitination
S557 Phosphorylation
S558 Phosphorylation
S559 Phosphorylation
S563 Phosphorylation
K573 Sumoylation
K573 Ubiquitination
S589 Phosphorylation
S594 Phosphorylation
S597 Phosphorylation
S598 Phosphorylation
T600 Phosphorylation
S602 Phosphorylation
S604 Phosphorylation
T618 Phosphorylation
S630 Phosphorylation
T632 Phosphorylation
S634 Phosphorylation
K641 Sumoylation
K656 Ubiquitination
T658 Phosphorylation
K670 Acetylation
K670 Ubiquitination
S674 Phosphorylation
S678 O-Glycosylation
S678 Phosphorylation
T679 Phosphorylation
S680 Phosphorylation
T681 Phosphorylation
T683 Phosphorylation
S684 O-Glycosylation
S684 Phosphorylation
R688 Methylation
Y706 Phosphorylation
Y744 Phosphorylation P12931 (SRC)

Research Backgrounds

Function:

Involved in the regulation of stress granule and P-body formation.

PTMs:

Thrombopoietin triggers the phosphorylation on tyrosine residues in a way that is dependent on MPL C-terminal domain.

Asymmetrically dimethylated. Probably methylated by PRMT1.

Subcellular Location:

Membrane>Peripheral membrane protein. Cytoplasm. Nucleus speckle. Cytoplasmic granule.
Note: Predominantly cytoplasmic but is also detected in nuclear speckles (PubMed:23209657). Component of cytoplasmic stress granules (PubMed:23209657). Inhibition of methylation alters nuclear localization (PubMed:25748791). Methylation does not seem to be required for localization to stress granules under stress conditions (PubMed:25748791).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.

Subunit Structure:

Interacts with MPL/TPOR and EPOR and dissociates after ligand stimulation. Interacts with DDX6, G3BP1, and ATXN2. Interacts with PRMT1. Interacts with CIC and ATXN1 (By similarity).

Family&Domains:

Belongs to the ataxin-2 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.