Product: ATPAF2 Antibody
Catalog: DF3805
Description: Rabbit polyclonal antibody to ATPAF2
Application: WB IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 35 KD; 33kD(Calculated).
Uniprot: Q8N5M1
RRID: AB_2836162

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(91%), Bovine(90%), Horse(91%), Sheep(80%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
ATPAF2 Antibody detects endogenous levels of total ATPAF2.
RRID:
AB_2836162
Cite Format: Affinity Biosciences Cat# DF3805, RRID:AB_2836162.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP synthase mitochondrial F1 complex assembly factor 2; ATP12; ATP12 homolog; ATP12p; LP3663; MC5DN1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8N5M1 ATPF2_HUMAN:

Widely expressed.

Sequence:
MWRSCLRLRDGGRRLLNRPAGGPSASMSPGPTIPSPARAYAPPTERKRFYQNVSITQGEGGFEINLDHRKLKTPQAKLFTVPSEALAIAVATEWDSQQDTIKYYTMHLTTLCNTSLDNPTQRNKDQLIRAAVKFLDTDTICYRVEEPETLVELQRNEWDPIIEWAEKRYGVEISSSTSIMGPSIPAKTREVLVSHLASYNTWALQGIEFVAAQLKSMVLTLGLIDLRLTVEQAVLLSRLEEEYQIQKWGNIEWAHDYELQELRARTAAGTLFIHLCSESTTVKHKLLKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
91
Horse
91
Chicken
91
Bovine
90
Sheep
80
Zebrafish
71
Xenopus
55
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N5M1 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S183 Phosphorylation
Y243 Phosphorylation
T266 Phosphorylation
T270 Phosphorylation

Research Backgrounds

Function:

May play a role in the assembly of the F1 component of the mitochondrial ATP synthase (ATPase).

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with ATP5F1A. Interacts with FMC1.

Family&Domains:

Belongs to the ATP12 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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