DDX19B Antibody | Affinity Biosciences

1/2

DDX19B Antibody - Western blot analysis of extracts from HT-29 cells, using DDX19B antibody.

DDX19B Antibody - Western blot analysis of extracts from various samples, using DDX19B Antibody.

Product:	DDX19B Antibody
Catalog:	DF3817
Description:	Rabbit polyclonal antibody to DDX19B
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Chicken
Mol.Wt.:	50 KD; 54kD(Calculated).
Uniprot:	Q9UMR2
RRID:	AB_2836174

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(91%), Chicken(82%)

Clonality:

Polyclonal

Specificity:

DDX19B Antibody detects endogenous levels of total DDX19B.

RRID:

AB_2836174
Cite Format: Affinity Biosciences Cat# DF3817, RRID:AB_2836174.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP-dependent RNA helicase DDX19B; DBP5; Dbp5, yeast, homolog of; DD19B_HUMAN; DDX19; DDX19B; DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B; DEAD box protein 19B; DEAD box RNA helicase DEAD5; DEAD-box protein 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog); DEAD/H BOX 19; helicase DEAD5; RNAh; TDBP; yeast Dbp5 homolog;

Immunogens

Immunogen:

Uniprot:

Q9UMR2(DD19B_HUMAN) >>Visit HPA database.

Gene(ID):

DDX19B(11269)

Sequence:

Q9UMR2 DD19B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQSLLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQENALPLMLAEPPQNLIAQSQSGTGKTAAFVLAMLSQVEPANKYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Chicken

Xenopus

Horse

Sheep

Dog

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UMR2 As Substrate

Q9UMR2

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S19	Phosphorylation		Uniprot
T48	Phosphorylation		Uniprot
S60	Phosphorylation		Uniprot
K64	Ubiquitination		Uniprot
S86	Phosphorylation		Uniprot
Y89	Phosphorylation		Uniprot
S90	Phosphorylation		Uniprot
K92	Ubiquitination		Uniprot
S93	Phosphorylation	O14757 (CHEK1)	Uniprot
S154	Phosphorylation		Uniprot
K187	Ubiquitination		Uniprot
K193	Ubiquitination		Uniprot
R199	Methylation		Uniprot
K202	Acetylation		Uniprot
K229	Acetylation		Uniprot
K287	Acetylation		Uniprot
K287	Ubiquitination		Uniprot
K308	Ubiquitination		Uniprot
T422	Phosphorylation		Uniprot
Y423	Phosphorylation		Uniprot
S445	Phosphorylation		Uniprot
T468	Phosphorylation		Uniprot
K476	Ubiquitination		Uniprot

Research Backgrounds

Q9UMR2_DD19B_HUMAN

Function:

ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.

Subcellular Location:

Cytoplasm. Nucleus>Nuclear pore complex. Nucleus membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Nuclear pore complex cytoplasmic fibrils.

Subunit Structure:

Interacts with NUP214 or RNA in a mutually exclusive manner.

Family&Domains:

The N-terminal extension helix acts as an autoinhibitory domain, preventing ATP hydrolysis, unless the N-terminus of the protein is displaced by RNA binding, allowing cleft closure to bring key side chains into position for catalysis.

Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

DDX19B Antibody - #DF3817