DDX50 Antibody - #DF3818
(3)
| Product: | DDX50 Antibody |
| Catalog: | DF3818 |
| Description: | Rabbit polyclonal antibody to DDX50 |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Rabbit, Dog |
| Mol.Wt.: | 82 KD; 83kD(Calculated). |
| Uniprot: | Q9BQ39 |
| RRID: | AB_2836175 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DDX50 Antibody detects endogenous levels of total DDX50.
RRID:
AB_2836175
Cite Format: Affinity Biosciences Cat# DF3818, RRID:AB_2836175.
Cite Format: Affinity Biosciences Cat# DF3818, RRID:AB_2836175.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
4933429B04Rik; ATP-dependent RNA helicase DDX50; Ddx50; DDX50_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 50; DEAD box protein 50; Gu beta; Gu-beta; GU2; GUB; MGC109605; MGC3199; Nucleolar protein Gu2; RH II; RH II/GuB; RNA helicase II / Gu beta;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9BQ39 DDX50_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BQ39 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S35 | Phosphorylation | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| T53 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K94 | Acetylation | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| T266 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| Y345 | Phosphorylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| K432 | Methylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| Y472 | Phosphorylation | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| K512 | Methylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| T519 | Phosphorylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| K606 | Acetylation | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| S683 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| S735 | Phosphorylation | Uniprot |
Research Backgrounds
Subcellular Location:
Nucleus>Nucleolus.
Subunit Structure:
Interacts with C1QBP.
Family&Domains:
Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Restrictive clause
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For Research Use Only.
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