DDX54 Antibody - #DF3820
(3)
| Product: | DDX54 Antibody |
| Catalog: | DF3820 |
| Description: | Rabbit polyclonal antibody to DDX54 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Mol.Wt.: | 98 KD; 99kD(Calculated). |
| Uniprot: | Q8TDD1 |
| RRID: | AB_2836177 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Polyclonal
Specificity:
DDX54 Antibody detects endogenous levels of total DDX54.
RRID:
AB_2836177
Cite Format: Affinity Biosciences Cat# DF3820, RRID:AB_2836177.
Cite Format: Affinity Biosciences Cat# DF3820, RRID:AB_2836177.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
2410015A15Rik; AI414901; ATP dependent RNA helicase DDX54; ATP-dependent RNA helicase DDX54; ATP-dependent RNA helicase DP97; DDX54; DDX54_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 54; DEAD box helicase 54; DEAD box protein 54; DEAD box RNA helicase 97 kDa; DP97; MGC2835; RGD1562539;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q8TDD1 DDX54_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAADKGPAAGPRSRAAMAQWRKKKGLRKRRGAASQARGSDSEDGEFEIQAEDDARARKLGPGRPLPTFPTSECTSDVEPDTREMVRAQNKKKKKSGGFQSMGLSYPVFKGIMKKGYKVPTPIQRKTIPVILDGKDVVAMARTGSGKTACFLLPMFERLKTHSAQTGARALILSPTRELALQTLKFTKELGKFTGLKTALILGGDRMEDQFAALHENPDIIIATPGRLVHVAVEMSLKLQSVEYVVFDEADRLFEMGFAEQLQEIIARLPGGHQTVLFSATLPKLLVEFARAGLTEPVLIRLDVDTKLNEQLKTSFFLVREDTKAAVLLHLLHNVVRPQDQTVVFVATKHHAEYLTELLTTQRVSCAHIYSALDPTARKINLAKFTLGKCSTLIVTDLAARGLDIPLLDNVINYSFPAKGKLFLHRVGRVARAGRSGTAYSLVAPDEIPYLLDLHLFLGRSLTLARPLKEPSGVAGVDGMLGRVPQSVVDEEDSGLQSTLEASLELRGLARVADNAQQQYVRSRPAPSPESIKRAKEMDLVGLGLHPLFSSRFEEEELQRLRLVDSIKNYRSRATIFEINASSRDLCSQVMRAKRQKDRKAIARFQQGQQGRQEQQEGPVGPAPSRPALQEKQPEKEEEEEAGESVEDIFSEVVGRKRQRSGPNRGAKRRREEARQRDQEFYIPYRPKDFDSERGLSISGEGGAFEQQAAGAVLDLMGDEAQNLTRGRQQLKWDRKKKRFVGQSGQEDKKKIKTESGRYISSSYKRDLYQKWKQKQKIDDRDSDEEGASDRRGPERRGGKRDRGQGASRPHAPGTPAGRVRPELKTKQQILKQRRRAQKLHFLQRGGLKQLSARNRRRVQELQQGAFGRGARSKKGKMRKRM
PTMs - Q8TDD1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| K312 | Methylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| S644 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| T753 | Phosphorylation | Uniprot | |
| S755 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| R802 | Methylation | Uniprot | |
| T814 | Phosphorylation | Uniprot | |
| R820 | Methylation | Uniprot | |
| R844 | Methylation | Uniprot | |
| R868 | Methylation | Uniprot |
Research Backgrounds
Function:
Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
Subcellular Location:
Nucleus>Nucleolus.
Subunit Structure:
Interacts in a hormone-dependent manner with nuclear receptors.
Family&Domains:
Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.
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