DDX55 Antibody - #DF3821

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Product: DDX55 Antibody
Catalog: DF3821
Description: Rabbit polyclonal antibody to DDX55
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 68 KD; 69kD(Calculated).
Uniprot: Q8NHQ9
RRID: AB_2836178

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(90%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
DDX55 Antibody detects endogenous levels of total DDX55.
RRID:
AB_2836178
Cite Format: Affinity Biosciences Cat# DF3821, RRID:AB_2836178.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent RNA helicase DDX55; ATP-dependent RNA helicase DDX55; DDX 55; ddx55; DDX55_HUMAN; DEAD box protein 55; FLJ16577; KIAA1595; MGC33209;

Immunogens

Immunogen:
Uniprot:

Q8NHQ9(DDX55_HUMAN) >>Visit HPA database.

Gene(ID):
DDX55(57696)
Sequence:
MEHVTEGSWESLPVPLHPQVLGALRELGFPYMTPVQSATIPLFMRNKDVAAEAVTGSGKTLAFVIPILEILLRREEKLKKSQVGAIIITPTRELAIQIDEVLSHFTKHFPEFSQILWIGGRNPGEDVERFKQQGGNIIVATPGRLEDMFRRKAEGLDLASCVRSLDVLVLDEADRLLDMGFEASINTILEFLPKQRRTGLFSATQTQEVENLVRAGLRNPVRVSVKEKGVAASSAQKTPSRLENYYMVCKADEKFNQLVHFLRNHKQEKHLVFFSTCACVEYYGKALEVLVKGVKIMCIHGKMKYKRNKIFMEFRKLQSGILVCTDVMARGIDIPEVNWVLQYDPPSNASAFVHRCGRTARIGHGGSALVFLLPMEESYINFLAINQKCPLQEMKPQRNTADLLPKLKSMALADRAVFEKGMKAFVSYVQAYAKHECNLIFRLKDLDFASLARGFALLRMPKMPELRGKQFPDFVPVDVNTDTIPFKDKIREKQRQKLLEQQRREKTENEGRRKFIKNKAWSKQKAKKEKKKKMNEKRKREEGSDIEDEDMEELLNDTRLLKKLKKGKITEEEFEKGLLTTGKRTIKTVDLGISDLEDDC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
90
Horse
90
Bovine
90
Sheep
90
Chicken
80
Dog
70
Xenopus
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NHQ9 As Substrate

Site PTM Type Enzyme
Y31 Phosphorylation
T33 Phosphorylation
S37 Phosphorylation
T39 Phosphorylation
K47 Ubiquitination
S81 Phosphorylation
T141 Phosphorylation
K152 Ubiquitination
R218 Methylation
S234 Phosphorylation
K237 Ubiquitination
T238 Phosphorylation
K316 Ubiquitination
S409 Phosphorylation
K444 Acetylation
K469 Ubiquitination
K517 Acetylation
K519 Acetylation
K523 Acetylation
S544 Phosphorylation
S594 Phosphorylation
C600 S-Nitrosylation

Research Backgrounds

Function:

Probable ATP-binding RNA helicase.

Family&Domains:

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.

Restrictive clause

 

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For Research Use Only.
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