Product: BCL2L12 Antibody
Catalog: DF3830
Description: Rabbit polyclonal antibody to BCL2L12
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 34 KD; 37kD(Calculated).
Uniprot: Q9HB09
RRID: AB_2836187

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(82%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BCL2L12 Antibody detects endogenous levels of total BCL2L12.
RRID:
AB_2836187
Cite Format: Affinity Biosciences Cat# DF3830, RRID:AB_2836187.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B2L12_HUMAN; BCL 2 like 12 (proline rich); BCL 2 like 12; BCL 2 like 12 isoform 1; Bcl 2 like 12 protein; Bcl 2 related proline rich protein; Bcl-2-like protein 12; Bcl-2-related proline-rich protein; BCL2 like 12 (proline rich); BCL2 like 12; BCL2 like 12 isoform 1; Bcl2 like 12 protein; Bcl2 related proline rich protein; Bcl2-L-12; BCL2L12; BPR; MGC120313; MGC120314; MGC120315;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9HB09 B2L12_HUMAN:

Expressed mainly in breast, thymus, prostate, fetal liver, colon, placenta, pancreas, small intestine, spinal cord, kidney, and bone marrow and to a lesser extent in many other tissues. Isoform 2 is primarily expressed in skeletal muscle.

Sequence:
MGRPAGLFPPLCPFLGFRPEACWERHMQIERAPSVPPFLRWAGYRPGPVRRRGKVELIKFVRVQWRRPQVEWRRRRWGPGPGASMAGSEELGLREDTLRVLAAFLRRGEAAGSPVPTPPRSPAQEEPTDFLSRLRRCLPCSLGRGAAPSESPRPCSLPIRPCYGLEPGPATPDFYALVAQRLEQLVQEQLKSPPSPELQGPPSTEKEAILRRLVALLEEEAEVINQKLASDPALRSKLVRLSSDSFARLVELFCSRDDSSRPSRACPGPPPPSPEPLARLALAMELSRRVAGLGGTLAGLSVEHVHSFTPWIQAHGGWEGILAVSPVDLNLPLD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Pig
91
Horse
91
Rabbit
82
Xenopus
57
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HB09 As Substrate

Site PTM Type Enzyme
S113 Phosphorylation
T117 Phosphorylation
S121 Phosphorylation
R144 Methylation
S156 Phosphorylation P49841 (GSK3B)
T171 Phosphorylation
K191 Ubiquitination
S192 Phosphorylation
S195 Phosphorylation
K206 Ubiquitination
K227 Ubiquitination
S236 Phosphorylation
S242 Phosphorylation
S243 Phosphorylation
S245 Phosphorylation
S273 Phosphorylation

Research Backgrounds

Tissue Specificity:

Expressed mainly in breast, thymus, prostate, fetal liver, colon, placenta, pancreas, small intestine, spinal cord, kidney, and bone marrow and to a lesser extent in many other tissues. Isoform 2 is primarily expressed in skeletal muscle.

Family&Domains:

Belongs to the Bcl-2 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.