Product: CLIP3 Antibody
Catalog: DF3878
Description: Rabbit polyclonal antibody to CLIP3
Application: WB IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 60 KD; 60kD(Calculated).
Uniprot: Q96DZ5
RRID: AB_2836231

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CLIP3 Antibody detects endogenous levels of total CLIP3.
RRID:
AB_2836231
Cite Format: Affinity Biosciences Cat# DF3878, RRID:AB_2836231.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1500005P14Rik; AI844915; CAP GLY domain containing linker protein 3; CAP-Gly domain-containing linker protein 3; CLIP 170 related 59 kDa protein; CLIP-170-related 59 kDa protein; clip3; CLIP3_HUMAN; CLIPR 59; CLIPR-59; CLIPR59; Cytoplasmic linker protein 170 related 59 kDa protein; Cytoplasmic linker protein 170-related 59 kDa protein; Restin like 1; RSNL1;

Immunogens

Immunogen:

A synthesized peptide derived from human CLIP3, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Sequence:
MTKTDPAPMAPPPRGEEEEEEEEDEPVPEAPSPTQERRQKPVVHPSAPAPLPKDYAFTFFDPNDPACQEILFDPQTTIPELFAIVRQWVPQVQHKIDVIGNEILRRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLSQQLLALGADVTLRSRWTNMNALHYAAYFDVPDLVRVLLKGARPRVVNSTCSDFNHGSALHIAASSLCLGAAKCLLEHGANPALRNRKGQVPAEVVPDPMDMSLDKAEAALVAKELRTLLEEAVPLSCALPKVTLPNYDNVPGNLMLSALGLRLGDRVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKAVDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKTPSSPSLGSLQQRDGAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQRIGGSTDSPGDSVGAKKVHQVTMTQPKRTFTTVRTPKDIASENSISRLLFCCWFPWMLRAEMQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
91
Rabbit
91
Xenopus
73
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics. May modulate the cellular compartmentalization of AKT kinase family and promote its cell membrane localization, thereby playing a role in glucose transport in adipocytes.

PTMs:

Palmitoylation by ZDHHC17 regulates association with the plasma membrane.

Subcellular Location:

Cell membrane>Lipid-anchor. Cytoplasm. Golgi apparatus>Golgi stack.
Note: Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).

References

1). Effect of CLIP3 Upregulation on Astrocyte Proliferation and Subsequent Glial Scar Formation in the Rat Spinal Cord via STAT3 Pathway After Injury. Journal of Molecular Neuroscience, 2017 (PubMed: 29218499) [IF=2.8]

Application: WB    Species: rat    Sample: spinal cord

Fig. 1 |Expression of CLIP3 is increased after spinal cord injury.a The BBB scale of rat hind limb locomotor at each time point after injury. b The spinal cord tissues removed from each time point after SCI. Protein immunoblots detected CLIP3 and GAPDH.

Application: IF/ICC    Species: rat    Sample: astrocytes of the adult spinal cord

Fig. 3 |The upregulation of CLIP3 protein in astrocytes of the adult spinal cord. The immunostaining for CLIP3 with GFAP (a–f), Iba1 (g–l), NeuN(m–n), and O4 (o–p).

Application: IHC    Species: rat    Sample: spinal cord

Fig. 2| The detection of CLIP3 in the sections of the rat spinal cord with immunohistochemistry staining. Sections were stained with specific antibody for CLIP3 under low-power views in sham-operated (a) and 3-day injury groups (b). Sections were stained with specific antibody for CLIP3 under higher power views in the ventral horn of white matter(c, d) and gry matter (e, f).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.