CLIP4 Antibody - #DF3879
(2)
(4)
| Product: | CLIP4 Antibody |
| Catalog: | DF3879 |
| Description: | Rabbit polyclonal antibody to CLIP4 |
| Application: | WB IHC IF/ICC |
| Cited expt.: | IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 76 KD; 76kD(Calculated). |
| Uniprot: | Q8N3C7 |
| RRID: | AB_2836232 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(91%), Sheep(82%), Rabbit(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
CLIP4 Antibody detects endogenous levels of total CLIP4.
RRID:
AB_2836232
Cite Format: Affinity Biosciences Cat# DF3879, RRID:AB_2836232.
Cite Format: Affinity Biosciences Cat# DF3879, RRID:AB_2836232.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
CAP GLY domain containing linker protein family member 4; CAP-Gly domain-containing linker protein 4; Clip4; CLIP4_HUMAN; Restin like 2; Restin-like protein 2; RSNL2;
Immunogens
Immunogen:
A synthesized peptide derived from human CLIP4, corresponding to a region within the internal amino acids.
Uniprot:
Gene(ID):
Sequence:
- Q8N3C7 CLIP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTIEDLPDFPLEGNPLFGRYPFIFSASDTPVIFSISAAPMPSDCEFSFFDPNDASCQEILFDPKTSVSELFAILRQWVPQVQQNIDIIGNEILKRGCNVNDRDGLTDMTLLHYTCKSGAHGIGDVETAVKFATQLIDLGADISLRSRWTNMNALHYAAYFDVPELIRVILKTSKPKDVDATCSDFNFGTALHIAAYNLCAGAVKCLLEQGANPAFRNDKGQIPADVVPDPVDMPLEMADAAATAKEIKQMLLDAVPLSCNISKAMLPNYDHVTGKAMLTSLGLKLGDRVVIAGQKVGTLRFCGTTEFASGQWAGIELDEPEGKNNGSVGKVQYFKCAPKYGIFAPLSKISKAKGRRKNITHTPSTKAAVPLIRSQKIDVAHVTSKVNTGLMTSKKDSASESTLSLPPGEELKTVTEKDVALLGSVSSCSSTSSLEHRQSYPKKQNAISSNKKTMSKSPSLSSRASAGLNSSATSTANNSRCEGELRLGERVLVVGQRLGTIRFFGTTNFAPGYWYGIELEKPHGKNDGSVGGVQYFSCSPRYGIFAPPSRVQRVTDSLDTLSEISSNKQNHSYPGFRRSFSTTSASSQKEINRRNAFSKSKAALRRSWSSTPTAGGIEGSVKLHEGSQVLLTSSNEMGTVRYVGPTDFASGIWLGLELRSAKGKNDGSVGDKRYFTCKPNHGVLVRPSRVTYRGINGSKLVDENC
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
91
Bovine
91
Pig
82
Sheep
82
Dog
82
Rabbit
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
References
1). miR-135b-5p promotes gastric carcinogenesis by targeting CLIP4-mediated JAK2/STAT3 signal pathway. Cellular signalling, 2024
(PubMed: 39121973)
[IF=4.4]
2). Endoplasmic reticulum stress-related CLIP4 plays a procarcinogenic role in hepatocellular carcinoma: an integrated analysis. BMC cancer, 2025
(PubMed: 39920578)
[IF=3.8]
Application: IHC Species: human Sample:
Fig. 1 Elevated CLIP4 expression correlates with poor prognosis in HCC patients. A The expression levels of CLIP4 were examined in 33 distinct types of cancer tissues alongside their corresponding adjacent tissues utilizing the TCGA database. B, C Unpaired (B) and paired samples (C) from the TCGA-LIHC dataset were used to analyze the mRNA expression of CLIP4 in liver cancer and normal tissues. D The relationship between CLIP4 expression and the OS of liver cancer patients was analyzed via the TCGA-LIHC dataset. E CLIP4 expression in different histological grade groups (TCGA). F, G Effects of CLIP4 expression on AFP level (F) and gender (G) (TCGA-LIHC). H Representative images of CLIP4 IHC staining intensity in liver cancer and adjacent tissues. I CLIP4 expression in HCC and adjacent nontumor tissues. J Effect of a history of hepatitis on CLIP4 expression. K, L IHC staining for CLIP4 in differentiation (K) and tumor size (L). M The OS of individuals with high expression (n = 19) or low expression (n = 14) of CLIP4 was analyzed via Kaplan‒Meier analysis
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For Research Use Only.
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