LYAR Antibody - #DF3913
| Product: | LYAR Antibody |
| Catalog: | DF3913 |
| Description: | Rabbit polyclonal antibody to LYAR |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Sheep |
| Mol.Wt.: | 44 KD; 44kD(Calculated). |
| Uniprot: | Q9NX58 |
| RRID: | AB_2836266 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3913, RRID:AB_2836266.
Fold/Unfold
Cell growth regulating nucleolar protein; Cell growth-regulating nucleolar protein; Likely ortholog of mouse Ly1 antibody reactive clone; Ly1 antibody reactive; Ly1 antibody reactive homolog (mouse); Ly1 antibody reactive homolog; LYAR; LYAR_HUMAN; ZC2HC2; ZLYAR;
Immunogens
- Q9NX58 LYAR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVKCISEDQKYGGKGYEGKTHKGDIKQQAWIQKISELIKRPNVSPKVRELLEQISAFDNVPRKKAKFQNWMKNSLKVHNESILDQVWNIFSEASNSEPVNKEQDQRPLHPVANPHAEISTKVPASKVKDAVEQQGEVKKNKRERKEERQKKRKREKKELKLENHQENSRNQKPKKRKKGQEADLEAGGEEVPEANGSAGKRSKKKKQRKDSASEEEAHVGAGKRKRRHSEVETDSKKKKMKLPEHPEGGEPEDDEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEHHRSEEELLVIFNKKISKNPTFKLLKDKVKLVK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NX58 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K61 | Acetylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K207 | Sumoylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| K247 | Acetylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| T314 | Phosphorylation | Uniprot | |
| Y340 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| T342 | Phosphorylation | Uniprot |
Research Backgrounds
Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'. Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity. In addition, inhibits NF-kappa-B-mediated expression of proinflammatory cytokines. Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).
Nucleus. Nucleus>Nucleolus. Cytoplasm. Cell projection>Cilium>Photoreceptor outer segment.
Note: Component of pre-ribosomal particles, including pre-40S, pre-60S and pre-90S (PubMed:24495227). Associated with cytoplasmic ribosomes, but not polysomes, as a component of the 60S subunit (PubMed:24990247). In the retina, predominantly expressed in photoreceptor outer segments (By similarity). In the nucleolus, colocalizes with nucleolin/NCL, therefore may reside in the dense fibrillar component (DFC) (By similarity).
Predominantly expressed in testis.
Interacts with PRMT5; this interaction is direct. Interacts with GNL2 and RPL23A. Interacts with nucleolin/NCL; this interaction is direct (By similarity). Interacts with phosphorylated IRF3; this interaction impairs IRF3 DNA-binding activity.
The N-terminal zinc-finger domains are required for the appropriate production of 28S rRNA and the formation of pre-60S particles.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.