CEP135 Antibody - #DF3916

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Product: CEP135 Antibody
Catalog: DF3916
Description: Rabbit polyclonal antibody to CEP135
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 134 KD; 133kD(Calculated).
Uniprot: Q66GS9
RRID: AB_2836269

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CEP135 Antibody detects endogenous levels of total CEP135.
RRID:
AB_2836269
Cite Format: Affinity Biosciences Cat# DF3916, RRID:AB_2836269.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

centrosomal protein 135 kDa; centrosomal protein 135kDa; Centrosomal protein 4; Centrosomal protein of 135 kDa; centrosome protein 4; centrosome protein cep135; Cep135; CEP4; CP135_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q66GS9(CP135_HUMAN) >>Visit HPA database.

Gene(ID):
CEP135(9662)
Sequence:
MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLIAHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Xenopus
73
Bovine
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q66GS9 As Substrate

Site PTM Type Enzyme
K51 Acetylation
Y86 Phosphorylation
K136 Ubiquitination
K148 Ubiquitination
T156 Phosphorylation
S184 Phosphorylation
S253 Phosphorylation
S258 Phosphorylation
S261 Phosphorylation
K311 Ubiquitination
K314 Ubiquitination
K333 Ubiquitination
K354 Acetylation
S356 Phosphorylation
S383 Phosphorylation
K389 Ubiquitination
K403 Ubiquitination
K409 Ubiquitination
S439 Phosphorylation
S441 Phosphorylation
K448 Ubiquitination
K482 Ubiquitination
S483 Phosphorylation
T488 Phosphorylation
K491 Ubiquitination
T502 Phosphorylation
K517 Ubiquitination
Y518 Phosphorylation
K527 Ubiquitination
K535 Ubiquitination
S548 Phosphorylation
K552 Ubiquitination
S554 Phosphorylation
T555 Phosphorylation
T557 Phosphorylation
K569 Ubiquitination
K571 Ubiquitination
K585 Ubiquitination
K591 Ubiquitination
S599 Phosphorylation
K603 Ubiquitination
K608 Ubiquitination
K635 Ubiquitination
K644 Ubiquitination
K651 Ubiquitination
K663 Ubiquitination
S688 Phosphorylation
K702 Ubiquitination
K727 Acetylation
K727 Ubiquitination
T728 Phosphorylation
K734 Ubiquitination
K749 Ubiquitination
T784 Phosphorylation
K804 Ubiquitination
K818 Methylation
K818 Ubiquitination
K857 Ubiquitination
K874 Ubiquitination
K951 Ubiquitination
K967 Ubiquitination
S976 Phosphorylation
K983 Ubiquitination
K988 Ubiquitination
S1014 Phosphorylation
S1016 Phosphorylation
K1031 Ubiquitination
K1043 Ubiquitination
K1085 Ubiquitination
K1097 Ubiquitination
T1121 Phosphorylation
S1126 Phosphorylation
S1130 Phosphorylation
S1132 Phosphorylation
S1134 Phosphorylation

Research Backgrounds

Function:

Involved in early centriole assembly/duplication/biogenesis/formation/. Required for centriole elongation. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner.

Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with DCTN2 (By similarity). Interacts with CEP250.

Family&Domains:

Belongs to the CEP135/TSGA10 family.

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