CEP70 Antibody | Affinity Biosciences

WB
IF/ICC

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CEP70 Antibody - Western blot analysis of extracts from Pc12, using CEP70 Antibody.

CEP70 Antibody - DF3920 staining Hela cells by IF/ICC.

Product:	CEP70 Antibody
Catalog:	DF3920
Description:	Rabbit polyclonal antibody to CEP70
Application:	WB IF/ICC
Reactivity:	Human, Rat
Prediction:	Bovine, Sheep
Mol.Wt.:	70 KD; 70kD(Calculated).
Uniprot:	Q8NHQ1
RRID:	AB_2836273

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Bovine(91%), Sheep(91%)

Clonality:

Polyclonal

Specificity:

CEP70 Antibody detects endogenous levels of total CEP70.

RRID:

AB_2836273
Cite Format: Affinity Biosciences Cat# DF3920, RRID:AB_2836273.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BITE; Centrosomal protein of 70 kDa; Cep70; CEP70_HUMAN; p10 binding protein; p10-binding protein;

Immunogens

Immunogen:

Uniprot:

Q8NHQ1(CEP70_HUMAN) >>Visit HPA database.

Gene(ID):

CEP70(80321)

Sequence:

Q8NHQ1 CEP70_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFPVAPKPQDSSQPSDRLMTEKQQEEAEWESINVLLMMHGLKPLSLVKRTDLKDLIIFDKQSSQRMRQNLKLLVEETSCQQNMIQELIETNQQLRNELQLEQSRAANQEQRANDLEQIMESVKSKIGELEDESLSRACHQQNKIKDLQKEQKTLQVKCQHYKKKRTEQEETIASLQMEVCRLKKEEEDRIVTQNRVFAYLCKRVPHTVLDRQLLCLIDYYESKIRKIHTQRQYKEDESQSEEENDYRNLDASPTYKGLLMSLQNQLKESKSKIDALSSEKLNLQKDLETRPTQHELRLYKQQVKKLEKALKKNVKLQELINHKKAEDTEKKDEPSKYNQQQALIDQRYFQVLCSINSIIHNPRAPVIIYKQTKGGVQNFNKDLVQDCGFEHLVPVIEMWADQLTSLKDLYKSLKTLSAELVPWLNLKKQDENEGIKVEDLLFIVDTMLEEVENKEKDSNMPHFQTLQAIVSHFQKLFDVPSLNGVYPRMNEVYTRLGEMNNAVRNLQELLELDSSSSLCVLVSTVGKLCRLINEDVNEQVMQVLGPEDLQSIIYKLEEHEEFFPAFQAFTNDLLEILEIDDLDAIVPAVKKLKVLSY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Zebrafish

Dog

Chicken

Pig

Horse

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NHQ1 As Substrate

Q8NHQ1

Site	PTM Type	Source
K53	Ubiquitination	Uniprot
K60	Ubiquitination	Uniprot
K123	Ubiquitination	Uniprot
K125	Ubiquitination	Uniprot
K157	Ubiquitination	Uniprot
K202	Ubiquitination	Uniprot
S238	Phosphorylation	Uniprot
S240	Phosphorylation	Uniprot
Y246	Phosphorylation	Uniprot
K256	Ubiquitination	Uniprot
K267	Ubiquitination	Uniprot
S271	Phosphorylation	Uniprot
K272	Ubiquitination	Uniprot
K280	Ubiquitination	Uniprot
K285	Ubiquitination	Uniprot
K300	Ubiquitination	Uniprot
K315	Ubiquitination	Uniprot
K323	Ubiquitination	Uniprot
K336	Ubiquitination	Uniprot
K370	Ubiquitination	Uniprot
K373	Ubiquitination	Uniprot
K427	Ubiquitination	Uniprot
K593	Ubiquitination	Uniprot

Research Backgrounds

Q8NHQ1_CEP70_HUMAN

Function:

Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localized at the center of the radial microtubule array in interphase and at the spindle poles during various stages of mitosis.

Subunit Structure:

Directly interacts with tubulin-gamma; this interaction determines centrosomal localization.

Family&Domains:

The coiled-coil domains may be important for tubulin-gamma-binding and hence for centrosomal localization.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CEP70 Antibody - #DF3920