CEP78 Antibody - #DF3922

(2)  
Product: CEP78 Antibody
Catalog: DF3922
Description: Rabbit polyclonal antibody to CEP78
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 80 KD; 76kD(Calculated).
Uniprot: Q5JTW2
RRID: AB_2836275

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
CEP78 Antibody detects endogenous levels of total CEP78.
RRID:
AB_2836275
Cite Format: Affinity Biosciences Cat# DF3922, RRID:AB_2836275.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5730599I05Rik; C9orf81; Centrosomal protein 78; Centrosomal protein 78kDa; Chromosome 9 open reading frame 81; D030027P05; FLJ12643; FLJ52093; IP63; MGC135040; RP11-336N8.5;

Immunogens

Immunogen:
Uniprot:

Q5JTW2(CEP78_HUMAN) >>Visit HPA database.

Gene(ID):
CEP78(84131)
Expression:
Q5JTW2 CEP78_HUMAN:

Widely expressed (PubMed:27588451, PubMed:27588452). Expressed in different retinal cell types with higher expression in cone compared to rod cells (at protein level) (PubMed:27588452).

Sequence:
MIDSVKLRRDSAADFFSHYEYLCALQNSVPLPAVRACLREGVLDFNADRLRGVDWAPLLSTLKINKDLPLVSIKSFFQPWLGDTGSDMNKFCRSRVPAIRYKDVTFQLCKALKGCLSISSVLKNLELNGLILRERDLTILAKGLNKSASLVHLSLANCPIGDGGLEIICQGIKSSITLKTVNFTGCNLTWQGADHMAKILKYQTMRRHEETWAESLRYRRPDLDCMAGLRRITLNCNTLIGDLGACAFADSLSEDLWLRALDLQQCGLTNEGAKALLEALETNTTLVVLDIRKNPLIDHSMMKAVIKKVLQNGRSAKSEYQWITSPSVKEPSKTAKQKRRTIILGSGHKGKATIRIGLATKKPVSSGRKHSLGKEYYAPAPLPPGVSGFLPWRTAERAKRHRGFPLIKTRDICNQLQQPGFPVTVTVESPSSSEVEEVDDSSESVHEVPEKTSIEQEALQEKLEECLKQLKEERVIRLKVDKRVSELEHENAQLRNINFSLSEALHAQSLTNMILDDEGVLGSIENSFQKFHAFLDLLKDAGLGQLATMAGIDQSDFQLLGHPQMTSTVSNPPKEEKKALEDEKPEPKQNALGQMQNIQFQKITGDARIPLPLDSFPVPVSTPEGLGTSSNNLGVPATEQRQESFEGFIARMCSPSPDATSGTGSQRKEEELSRNSRSSSEKKTKTESH

PTMs - Q5JTW2 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
S60 Phosphorylation
T61 Phosphorylation
K66 Ubiquitination
K90 Ubiquitination
K102 Ubiquitination
T138 Phosphorylation
S147 Phosphorylation
S149 Phosphorylation
S174 Phosphorylation
S175 Phosphorylation
K198 Ubiquitination
K201 Ubiquitination
K303 Ubiquitination
K317 Ubiquitination
S318 Phosphorylation
Y320 Phosphorylation
S325 Phosphorylation
S327 Phosphorylation
K329 Ubiquitination
K336 Ubiquitination
S346 Phosphorylation
K349 Ubiquitination
K361 Acetylation
K361 Ubiquitination
S371 Phosphorylation
K374 Ubiquitination
Y376 Phosphorylation
Y377 Phosphorylation
K408 Ubiquitination
K462 Ubiquitination
K468 Ubiquitination
S485 Phosphorylation
K578 Ubiquitination
K584 Ubiquitination
K602 Ubiquitination
S644 Phosphorylation
S654 Phosphorylation
S656 Phosphorylation
K682 Ubiquitination

Research Backgrounds

Function:

May be required for efficient PLK4 centrosomal localization and PLK4-induced overduplication of centrioles. May play a role in cilium biogenesis.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Mainly localizes at the centriolar wall, but also found in the pericentriolar material (PubMed:27246242). Expressed in photoreceptor inner segment (PubMed:27588452).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Expressed in different retinal cell types with higher expression in cone compared to rod cells (at protein level).

Subunit Structure:

Interacts with PLK4. Interacts with FAM161A.

Family&Domains:

Belongs to the CEP78 family.

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