HUCE1 Antibody - #DF3926

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Product: HUCE1 Antibody
Catalog: DF3926
Description: Rabbit polyclonal antibody to HUCE1
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 51 KD; 51kD(Calculated).
Uniprot: O43159
RRID: AB_2836279

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HUCE1 Antibody detects endogenous levels of total HUCE1.
RRID:
AB_2836279
Cite Format: Affinity Biosciences Cat# DF3926, RRID:AB_2836279.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cerebral protein 1; hypothetical protein LOC23378; KIAA0409; NML; Nucleomethylin; Ribosomal RNA processing 8 methyltransferase homolog; Ribosomal RNA processing 8, methyltransferase, homolog (yeast); Ribosomal RNA processing protein 8; Ribosomal RNA-processing protein 8; RRP 8; RRP8; RRP8 methyltransferase homolog; RRP8_HUMAN;

Immunogens

Immunogen:
Uniprot:

O43159(RRP8_HUMAN) >>Visit HPA database.

Gene(ID):
RRP8(23378)
Sequence:
MFEEPEWAEAAPVAAGLGPVISRPPPAASSQNKGSKRRQLLATLRALEAASLSQHPPSLCISDSEEEEEERKKKCPKKASFASASAEVGKKGKKKCQKQGPPCSDSEEEVERKKKCHKQALVGSDSAEDEKRKRKCQKHAPINSAQHLDNVDQTGPKAWKGSTTNDPPKQSPGSTSPKPPHTLSRKQWRNRQKNKRRCKNKFQPPQVPDQAPAEAPTEKTEVSPVPRTDSHEARAGALRARMAQRLDGARFRYLNEQLYSGPSSAAQRLFQEDPEAFLLYHRGFQSQVKKWPLQPVDRIARDLRQRPASLVVADFGCGDCRLASSIRNPVHCFDLASLDPRVTVCDMAQVPLEDESVDVAVFCLSLMGTNIRDFLEEANRVLKPGGLLKVAEVSSRFEDVRTFLRAVTKLGFKIVSKDLTNSHFFLFDFQKTGPPLVGPKAQLSGLQLQPCLYKRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43159 As Substrate

Site PTM Type Enzyme
T43 Phosphorylation
S51 Phosphorylation
S53 Phosphorylation
S58 Phosphorylation
S62 Phosphorylation
S64 Phosphorylation
K78 Acetylation
S80 Phosphorylation
S83 Phosphorylation
S85 Phosphorylation
S104 Phosphorylation
S106 Phosphorylation
S124 Phosphorylation
S126 Phosphorylation
K157 Sumoylation
K157 Ubiquitination
S162 Phosphorylation
T163 Phosphorylation
S171 Phosphorylation
S174 Phosphorylation
T175 Phosphorylation
S176 Phosphorylation
S184 Phosphorylation
K201 Sumoylation
S223 Phosphorylation
S286 Phosphorylation
R401 Methylation
K431 Ubiquitination
K440 Sumoylation
K440 Ubiquitination
K454 Sumoylation

Research Backgrounds

Function:

Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.

Subcellular Location:

Nucleus>Nucleolus.
Note: Localizes at rDNA locus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.

Family&Domains:

Belongs to the methyltransferase superfamily. RRP8 family.

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