KIF4A Antibody - #DF3941

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Product: KIF4A Antibody
Catalog: DF3941
Description: Rabbit polyclonal antibody to KIF4A
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 140 KD; 140kD(Calculated).
Uniprot: O95239
RRID: AB_2836294

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
KIF4A Antibody detects endogenous levels of total KIF4A.
RRID:
AB_2836294
Cite Format: Affinity Biosciences Cat# DF3941, RRID:AB_2836294.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Chromokinesin; Chromokinesin-A; Chromosome associated kinesin KIF4A; Chromosome-associated kinesin KIF4A; FLJ12530; FLJ12655; FLJ14204; FLJ20631; HSA271784; KIF4; KIF4 G1; KIF4A; KIF4A_HUMAN; KIF4G1; Kinesin family member 4A;

Immunogens

Immunogen:
Uniprot:

O95239(KIF4A_HUMAN) >>Visit HPA database.

Gene(ID):
KIF4A(24137)
Expression:
O95239 KIF4A_HUMAN:

Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.

Sequence:
MKEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLFKEIDKKSDFEFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISLEQRKKSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGDDKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQVLLLQAHGGTLPGSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRQHAACKLDLQKLVETLEDQELKENVEIICNLQQLITQLSDETVACMAAAIDTAVEQEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKALALKEALARKMTQNDSQLQPIQYQYQDNIKELELEVINLQKEKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLNEQSKLLKLKESTERTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQREVADKRKETQSRGMEGTAARVKNWLGNEIEVMVSTEEAKRHLNDLLEDRKILAQDVAQLKEKKESGENPPPKLRRRTFSLTEVRGQVSESEDSITKQIESLETEMEFRSAQIADLQQKLLDAESEDRPKQRWENIATILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCADMQKMLFEERNHFAEIETELQAELVRMEQQHQEKVLYLLSQLQQSQMAEKQLEESVSEKEQQLLSTLKCQDEELEKMREVCEQNQQLLRENEIIKQKLTLLQVASRQKHLPKDTLLSPDSSFEYVPPKPKPSRVKEKFLEQSMDIEDLKYCSEHSVNEHEDGDGDDDEGDDEEWKPTKLVKVSRKNIQGCSCKGWCGNKQCGCRKQKSDCGVDCCCDPTKCRNRQQGKDSLGTVERTQDSEGSFKLEDPTEVTPGLSFFNPVCATPNSKILKEMCDVEQVLSKKTPPAPSPFDLPELKHVATEYQENKAPGKKKKRALASNTSFFSGCSPIEEEAH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
90
Chicken
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95239 As Substrate

Site PTM Type Enzyme
K129 Ubiquitination
K158 Ubiquitination
K172 Ubiquitination
S197 Phosphorylation
T199 Phosphorylation
T203 Phosphorylation
S207 Phosphorylation
S209 Phosphorylation
S210 Phosphorylation
S234 Phosphorylation
K235 Ubiquitination
S244 Phosphorylation
K251 Ubiquitination
K258 Ubiquitination
K281 Acetylation
K282 Acetylation
Y288 Phosphorylation
K292 Ubiquitination
T294 Phosphorylation
S394 Phosphorylation
K401 Sumoylation
K401 Ubiquitination
S407 Phosphorylation
T423 Phosphorylation
K429 Ubiquitination
K433 Ubiquitination
K444 Ubiquitination
K449 Ubiquitination
K460 Sumoylation
S507 Phosphorylation
S508 Phosphorylation
K542 Sumoylation
K542 Ubiquitination
T544 Phosphorylation
K624 Acetylation
S634 Phosphorylation
K635 Ubiquitination
K670 Ubiquitination
K676 Ubiquitination
Y684 Phosphorylation
K688 Ubiquitination
K715 Ubiquitination
K772 Sumoylation
K782 Ubiquitination
T799 Phosphorylation Q96GD4 (AURKB)
S801 Phosphorylation
S810 Phosphorylation
S812 Phosphorylation
S815 Phosphorylation
T817 Phosphorylation
K840 Ubiquitination
K851 Ubiquitination
K868 Acetylation
Y869 Phosphorylation
S876 Phosphorylation
S877 Phosphorylation
K883 Ubiquitination
T893 Phosphorylation
S894 Phosphorylation
S941 Phosphorylation
K946 Ubiquitination
S951 Phosphorylation
S953 Phosphorylation
K955 Ubiquitination
S961 Phosphorylation
K964 Ubiquitination
K993 Ubiquitination
T995 Phosphorylation
S1001 Phosphorylation
T1010 Phosphorylation
S1013 Phosphorylation
S1016 Phosphorylation
S1017 Phosphorylation
Y1020 Phosphorylation
S1028 Phosphorylation
S1038 Phosphorylation
S1048 Phosphorylation
S1051 Phosphorylation
K1071 Sumoylation
K1095 Ubiquitination
T1115 Phosphorylation
S1126 Phosphorylation
T1133 Phosphorylation
S1136 Phosphorylation
S1139 Phosphorylation
T1146 Phosphorylation
T1149 Phosphorylation
T1161 Phosphorylation
S1164 Phosphorylation
S1178 Phosphorylation
T1181 Phosphorylation
S1186 Phosphorylation
Y1200 Phosphorylation
S1216 Phosphorylation
S1219 Phosphorylation
S1222 Phosphorylation
S1225 Phosphorylation

Research Backgrounds

Function:

Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.

Subcellular Location:

Nucleus matrix. Cytoplasm>Cytoskeleton>Spindle. Midbody. Chromosome.
Note: Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.

Subunit Structure:

Interacts with unphosphorylated PRC1 during late mitosis.

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Chromokinesin subfamily.

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