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ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1541]
LSD1/AOF2 antibody detects endogenous levels of total LSD1/AOF2.
Cite Format: Affinity Biosciences Cat# BF0551, RRID:AB_2833566.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Amine oxidase (flavin containing) domain 2; Amine oxidase, flavin containing, 2; AOF2; BHC110; BRAF35 HDAC complex protein BHC110; BRAF35-HDAC complex protein BHC110; BRAF35/HDAC complex, 110-kD subunit; CPRF; EC1; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; KDM 1; KDM1; Kdm1a; KDM1A_HUMAN; KIAA0601; LSD 1; LSD1; Lysine (K) specific demethylase 1; Lysine (K) specific demethylase 1A; Lysine demethylase 1A; Lysine specific histone demethylase 1; Lysine specific histone demethylase 1A; Lysine-specific demethylase 1; Lysine-specific demethylase 1A; Lysine-specific histone demethylase 1A;



Purified recombinant fragment of human LSD1/AOF2 expressed in E. Coli.


Ubiquitously expressed.

The amine oxidase domain 2 (AOF2) gene encodes a nuclear protein (LSD1, ~95kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serve as a docking module for the stabilization of the associated corepressor complex (es) on chromatin.

PTMs - O60341 As Substrate

Site PTM Type Enzyme
T59 Phosphorylation
S69 Phosphorylation
S80 Phosphorylation
T104 Phosphorylation
T110 Phosphorylation
S111 Phosphorylation
K114 Methylation
K117 Sumoylation
S126 Phosphorylation
S131 Phosphorylation P68400 (CSNK2A1)
Y135 Phosphorylation
Y136 Phosphorylation
S137 Phosphorylation P68400 (CSNK2A1)
S166 Phosphorylation
S172 Phosphorylation
R187 Methylation
K268 Ubiquitination
K271 Ubiquitination
T275 Phosphorylation
K280 Ubiquitination
S299 Phosphorylation
T305 Phosphorylation
K322 Methylation
K322 Ubiquitination
T335 Phosphorylation
S346 Phosphorylation
K347 Ubiquitination
K355 Ubiquitination
K359 Ubiquitination
Y363 Phosphorylation
K372 Ubiquitination
K374 Ubiquitination
K424 Sumoylation
K424 Ubiquitination
K432 Acetylation
K432 Ubiquitination
K433 Acetylation
K436 Acetylation
K436 Ubiquitination
K442 Sumoylation
K442 Ubiquitination
K447 Ubiquitination
K452 Sumoylation
K452 Ubiquitination
K456 Ubiquitination
K463 Ubiquitination
S466 Phosphorylation
K469 Ubiquitination
K481 Ubiquitination
K492 Ubiquitination
K503 Ubiquitination
K507 Ubiquitination
K585 Sumoylation
K585 Ubiquitination
T588 Phosphorylation
S611 Phosphorylation
K617 Ubiquitination
K631 Ubiquitination
K647 Ubiquitination
K661 Ubiquitination
S683 Phosphorylation P49841 (GSK3B)
S687 Phosphorylation
K732 Ubiquitination
K744 Ubiquitination
R838 Methylation
T841 Phosphorylation
S849 Phosphorylation
S851 Phosphorylation

Research Backgrounds


Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.


Polyubiquitinated by JADE2; which leads to its proteasomal degradation.

Subcellular Location:


Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of a histone demethylase complex with RCOR1. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Interacts with INSM1 (via N-terminus). Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with SNAI1 (via SNAG domain). Interacts (via AOD/Tower domain) with JADE2 (via C-terminus). Interacts with ESRRB; co-occupes the core set of ESRRB targets (By similarity).


The SWIRM domain may act as an anchor site for a histone tail.

Belongs to the flavin monoamine oxidase family.

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