CLASP1 Antibody - #DF3949

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Product: CLASP1 Antibody
Catalog: DF3949
Description: Rabbit polyclonal antibody to CLASP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 169 KD; 169kD(Calculated).
Uniprot: Q7Z460
RRID: AB_2836302

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
CLASP1 Antibody detects endogenous levels of total CLASP1.
RRID:
AB_2836302
Cite Format: Affinity Biosciences Cat# DF3949, RRID:AB_2836302.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1700030C23Rik; 5730583A19Rik; B130045P17Rik; CLAP1_HUMAN; clasp1; CLIP associating protein 1; CLIP associating protein CLASP1; CLIP-associating protein 1; Cytoplasmic linker associated protein 1; Cytoplasmic linker-associated protein 1; DKFZp686D1968; DKFZp686H2039; FLJ33821; FLJ41222; hOrbit1; KIAA0622; MAST1; MGC131895; mKIAA0622; Multiple asters 1; Multiple asters homolog 1; Protein Orbit homolog 1;

Immunogens

Immunogen:
Uniprot:

Q7Z460(CLAP1_HUMAN) >>Visit HPA database.

Gene(ID):
CLASP1(23332)
Sequence:
MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQTMLDKLVDGLATSWVNSSNYKVVLLGMDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKGLPQSRLNVIFTKFDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTRRLGSSTLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRSLLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPNSAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAETIKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQESLNRPLSAKRSPTGSTTSRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFSSAAALPPGSYASLGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQRSRSANPAGAGSRSSSPGKLLGSGYGGLTGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLARSSRIPRPSMSQGCSRDTSRESSRDTSPARGFPPLDRFGLGQPGRIPGSVNAMRVLSTSTDLEAAVADALKKPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHYLRQTEDVAEVLNHCASSNWSERKEGLLGLQNLLKSQRTLSRVELKRLCEIFTRMFADPHSKRVFSMFLETLVDFIIIHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRDSFPFDQQFNILMRFIVDQTQTPNLKVKVAILKYIESLARQMDPTDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQIVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLKNSSNTSVGSPSNTIGRTPSRHTSSRTSPLTSPTNCSHGGLSPSRLWGWSADGLAKHPPPFSQPNSIPTAPSHKALRRSYSPSMLDYDTENLNSEEIYSSLRGVTEAIEKFSFRSQEDLNEPIKRDGKKECDIVSRDGGAASPATEGRGGSEVEGGRTALDNKTSLLNTQPPRAFPGPRARDYNPYPYSDAINTYDKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEERKGALLELLKITREDSLGVWEEHFKTILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEAHKDSHKEVVRAAEEAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERIAKESLLQLLVDIIPGLLQGYDNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Chicken
100
Rabbit
100
Pig
91
Bovine
91
Sheep
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z460 As Substrate

Site PTM Type Enzyme
S214 Phosphorylation
K228 Ubiquitination
S246 Phosphorylation
S254 Phosphorylation
T274 Phosphorylation
T275 Phosphorylation
S280 Phosphorylation
S281 Phosphorylation
S285 Phosphorylation
K304 Ubiquitination
K326 Ubiquitination
K335 Ubiquitination
S485 Phosphorylation
S545 Phosphorylation
S552 Phosphorylation
S555 Phosphorylation
S557 Phosphorylation
S558 Phosphorylation
S559 Phosphorylation
S562 Phosphorylation
S568 Phosphorylation
S572 Phosphorylation
T574 Phosphorylation
S576 Phosphorylation
T578 Phosphorylation
T586 Phosphorylation
S588 Phosphorylation
S590 Phosphorylation
S594 Phosphorylation
S598 Phosphorylation
S600 Phosphorylation
S609 Phosphorylation
S612 Phosphorylation
S624 Phosphorylation
S633 Phosphorylation
Y634 Phosphorylation
S636 Phosphorylation
T642 Phosphorylation
S646 Phosphorylation
S647 Phosphorylation
S649 Phosphorylation
T651 Phosphorylation
S655 Phosphorylation
T656 Phosphorylation
R660 Methylation
R662 Methylation
S674 Phosphorylation
S676 Phosphorylation
S684 Phosphorylation
S686 Phosphorylation
S687 Phosphorylation
S688 Phosphorylation
S695 Phosphorylation
Y697 Phosphorylation
T701 Phosphorylation
S704 Phosphorylation
S705 Phosphorylation
R706 Methylation
T711 Phosphorylation
S713 Phosphorylation
S714 Phosphorylation
S723 Phosphorylation
S727 Phosphorylation
T730 Phosphorylation
S731 Phosphorylation
S740 Phosphorylation
S749 Phosphorylation
S760 Phosphorylation
T764 Phosphorylation
S765 Phosphorylation
S787 Phosphorylation
S795 Phosphorylation
T796 Phosphorylation
S797 Phosphorylation
T798 Phosphorylation
S823 Phosphorylation
S829 Phosphorylation
C835 S-Nitrosylation
S836 Phosphorylation
R843 Methylation
R872 Methylation
K873 Ubiquitination
K1000 Ubiquitination
T1059 Phosphorylation
T1068 Phosphorylation
T1074 Phosphorylation
K1075 Ubiquitination
K1082 Ubiquitination
S1084 Phosphorylation
S1085 Phosphorylation
S1088 Phosphorylation
S1091 Phosphorylation
S1093 Phosphorylation
T1095 Phosphorylation
T1099 Phosphorylation
T1112 Phosphorylation
S1113 Phosphorylation
S1123 Phosphorylation
Y1179 Phosphorylation
K1191 Ubiquitination
S1193 Phosphorylation
S1196 Phosphorylation
C1212 S-Nitrosylation
S1223 Phosphorylation
T1226 Phosphorylation
R1229 Methylation
T1245 Phosphorylation
Y1264 Phosphorylation
Y1267 Phosphorylation
Y1269 Phosphorylation
T1275 Phosphorylation
Y1276 Phosphorylation
K1278 Ubiquitination
K1282 Ubiquitination
T1354 Phosphorylation
S1399 Phosphorylation
Y1517 Phosphorylation
T1523 Phosphorylation

Research Backgrounds

Function:

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle. Golgi apparatus>trans-Golgi network.
Note: Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CLIP2, ERC1, MAPRE1, MAPRE3, microtubules, PHLDB2 and RSN. The interaction with ERC1 may be mediated by PHLDB2. Interacts with GCC2; recruits CLASP1 to Golgi membranes. Interacts with MACF1 (By similarity).

Family&Domains:

Belongs to the CLASP family.

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