RCL Antibody - #DF3951

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Product: RCL Antibody
Catalog: DF3951
Description: Rabbit polyclonal antibody to RCL
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 22 KD; 19kD(Calculated).
Uniprot: O43598
RRID: AB_2836304

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(80%), Bovine(90%), Sheep(90%), Rabbit(80%), Dog(90%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
RCL Antibody detects endogenous levels of total RCL.
RRID:
AB_2836304
Cite Format: Affinity Biosciences Cat# DF3951, RRID:AB_2836304.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2' deoxynucleoside 5' phosphate N hydrolase 1; BC048355; c Myc responsive; c Myc responsive protein Rcl; c-Myc-responsive protein Rcl; C6orf108; C76683; Chromosome 6 open reading frame 108; Deoxyribonucleoside 5' monophosphate N glycosidase; Deoxyribonucleoside 5''-monophosphate N-glycosidase; dJ330M21.3; DNPH1; MGC54855; Putative c Myc responsive; Rcl; RCL_HUMAN; RP3 330M21.3;

Immunogens

Immunogen:
Uniprot:

O43598(DNPH1_HUMAN) >>Visit HPA database.

Gene(ID):
DNPH1(10591)
Expression:
O43598 DNPH1_HUMAN:

Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers.

Sequence:
MAAAMVPGRSESWERGEPGRPALYFCGSIRGGREDRTLYERIVSRLRRFGTVLTEHVAAAELGARGEEAAGGDRLIHEQDLEWLQQADVVVAEVTQPSLGVGYELGRAVAFNKRILCLFRPQSGRVLSAMIRGAADGSRFQVWDYEEGEVEALLDRYFEADPPGQVAASPDPTT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
90
Sheep
90
Dog
90
Xenopus
82
Pig
80
Rabbit
80
Zebrafish
55
Horse
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43598 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S10 Phosphorylation
S12 Phosphorylation
S28 Phosphorylation
R30 Methylation
R36 Methylation
R41 Methylation
R48 Methylation
R65 Methylation
T95 Phosphorylation
S98 Phosphorylation
K113 Ubiquitination
S123 Phosphorylation
S128 Phosphorylation
R132 Methylation
S138 Phosphorylation
S169 Phosphorylation
T173 Phosphorylation
T174 Phosphorylation

Research Backgrounds

Function:

Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers.

Subunit Structure:

Monomer and homodimer.

Family&Domains:

Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family.

Restrictive clause

 

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For Research Use Only.
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