Product: CCDC45 Antibody
Catalog: DF3956
Description: Rabbit polyclonal antibody to CCDC45
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 95 KD; 95kD(Calculated).
Uniprot: Q96GE4
RRID: AB_2836309

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
CCDC45 Antibody detects endogenous levels of total CCDC45.
RRID:
AB_2836309
Cite Format: Affinity Biosciences Cat# DF3956, RRID:AB_2836309.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCDC45; Centrosomal protein 95kDa; Centrosomal protein of 95 kDa; Cep95; CEP95_HUMAN; Coiled coil domain containing 45; Coiled-coil domain-containing protein 45;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAGSDAEWVTIANNLLFKCHIHLRIHELQDCDANVFIALYQSILGEKVPDLIVIPRSQEDDAHNVQAVIDSLALDYLQVSLSHITGENIVKGDKESIKNLLEIFDGLLEYLTERISETSHEKSETEQYFKESDRGERLEEPESTKESKSSWKRVSFGRCSLSSEMLGPSWDGDEAESTGEIIRLGDTAHTFSLRSNGAQCPNEMLSKKALASPSSKSHEDMLYPPSVLSKSRTSFVEDTETLSVSGIPNARKLGEPIRAAIPLHPPYHPSEPRAPCPIGKEYLHSSHCSPAVNSTGEHTEFSGDLDDGLFLISKLPKGSKWEVYPAQVQGPRTRKPPKGKRNENRATASSCNSPFPQRPRKRLTEQELHDVSEKLSQRLSELDWMLKSALGDRIKEKTDHKEENTGNEEVEDGTEETLSQHSDGIVEYGPKKSRPGLSMRRKPPYRSHSLSPSPVNKHKQFHLERKRQRKPRETDVRQFQAQAFTEAFERELRRHKVQENIGPLRIHEKEEETEKIYRGEAVRKGTPECSQPWKIYSRKTTTQSLRGGLPKPNKAVPMKVSEHSLLPLMLEQFPFLYVSGPTLSKMWKQQIAQVEQLKKEACRENRSKKKLQDEIEEALRRHDLLTTLVKKEYEHNKRLQDFKDCIRRQRLTQSKIKENRQQIVRARKYYDDYRVQLCAKMMRMRTREEMIFKKLFEEGLNIQKQRLRDLRNYAKEKRDEQRRRHQDELDSMENYYKDQFSLLAEAISQEHQELKAREKSQAQTLHKVKRELRSKMEKEIQQLQDMITQNDDDVFFRELEAERFRSRLQLASFQYSKSPSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Pig
91
Dog
82
Rabbit
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96GE4 As Substrate

Site PTM Type Enzyme
K130 Ubiquitination
K145 Ubiquitination
S155 Phosphorylation
S212 Phosphorylation
S217 Phosphorylation
S226 Phosphorylation
S229 Phosphorylation
K230 Ubiquitination
S243 Phosphorylation
K252 Ubiquitination
K314 Ubiquitination
K320 Ubiquitination
T333 Phosphorylation
R341 Methylation
S353 Phosphorylation
K374 Ubiquitination
K387 Sumoylation
K387 Ubiquitination
S388 Phosphorylation
S419 Phosphorylation
S422 Phosphorylation
K442 Methylation
S447 Phosphorylation
S449 Phosphorylation
S451 Phosphorylation
S453 Phosphorylation
K496 Ubiquitination
K524 Ubiquitination
T526 Phosphorylation
K534 Ubiquitination
S544 Phosphorylation
K588 Ubiquitination
K598 Ubiquitination
K610 Ubiquitination
K643 Ubiquitination
K668 Ubiquitination
K680 Ubiquitination
K694 Ubiquitination
K704 Ubiquitination
Y735 Phosphorylation
Y736 Phosphorylation
K755 Ubiquitination
K759 Ubiquitination
K767 Ubiquitination
S812 Phosphorylation
K817 Ubiquitination
S818 Phosphorylation
S820 Phosphorylation

Research Backgrounds

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

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For Research Use Only.
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