NCAPH Antibody - #DF3967

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Product: NCAPH Antibody
Catalog: DF3967
Description: Rabbit polyclonal antibody to NCAPH
Application: WB IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human
Prediction: Pig, Horse, Rabbit
Mol.Wt.: 83 KD; 83kD(Calculated).
Uniprot: Q15003
RRID: AB_2836320

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(83%), Horse(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
NCAPH Antibody detects endogenous levels of total NCAPH.
RRID:
AB_2836320
Cite Format: Affinity Biosciences Cat# DF3967, RRID:AB_2836320.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Barren Drosophila Homolog of 1; Barren homolog protein 1; BRRN; BRRN1; CAP H; CAPH; Chromosome associated protein H; Chromosome-associated protein H; CND2_HUMAN; Condensin complex subunit 2; Condensin subunit 2; Condensin-2 complex subunit H2; Condesin; HCAP H; hCAP-H; KIAA0074; mCAP H; MGC4616; Ncaph; Non SMC condensin I complex subunit H; Non-SMC condensin I complex subunit H; XCAP H homolog; XCAP-H homolog;

Immunogens

Immunogen:

A synthesized peptide derived from human NCAPH, corresponding to a region within the internal amino acids.

Uniprot:

Q15003(CND2_HUMAN) >>Visit HPA database.

Gene(ID):
NCAPH(23397)
Expression:
Q15003 CND2_HUMAN:

Widely expressed at low level. Expressed in proliferating cells.

Sequence:
MGPPGPALPATMNNSSSETRGHPHSASSPSERVFPMPLPRKAPLNIPGTPVLEDFPQNDDEKERLQRRRSRVFDLQFSTDSPRLLASPSSRSIDISATIPKFTNTQITEHYSTCIKLSTENKITTKNAFGLHLIDFMSEILKQKDTEPTNFKVAAGTLDASTKIYAVRVDAVHADVYRVLGGLGKDAPSLEEVEGHVADGSATEMGTTKKAVKPKKKHLHRTIEQNINNLNVSEADRKCEIDPMFQKTAASFDECSTAGVFLSTLHCQDYRSELLFPSDVQTLSTGEPLELPELGCVEMTDLKAPLQQCAEDRQICPSLAGFQFTQWDSETHNESVSALVDKFKKNDQVFDINAEVDESDCGDFPDGSLGDDFDANDEPDHTAVGDHEEFRSWKEPCQVQSCQEEMISLGDGDIRTMCPLLSMKPGEYSYFSPRTMSMWAGPDHWRFRPRRKQDAPSQSENKKKSTKKDFEIDFEDDIDFDVYFRKTKAATILTKSTLENQNWRATTLPTDFNYNVDTLVQLHLKPGTRLLKMAQGHRVETEHYEEIEDYDYNNPNDTSNFCPGLQAADSDDEDLDDLFVGPVGNSDLSPYPCHPPKTAQQNGDTPEAQGLDITTYGESNLVAEPQKVNKIEIHYAKTAKKMDMKKLKQSMWSLLTALSGKEADAEANHREAGKEAALAEVADEKMLSGLTKDLQRSLPPVMAQNLSIPLAFACLLHLANEKNLKLEGTEDLSDVLVRQGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Pig
83
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.

PTMs:

Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation (By similarity).

Subcellular Location:

Nucleus. Cytoplasm. Chromosome.
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed at low level. Expressed in proliferating cells.

Family&Domains:

Belongs to the CND2 (condensin subunit 2) family.

References

1). NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway. CELL CYCLE, 2022 (PubMed: 34974790) [IF=3.4]

Application: WB    Species: Mouse    Sample: SW780 cells

Figure 2. NCAPH promotes the cell proliferation of BC cells. (a,b) The mRNA and protein levels of NCAPH in SW780 cells transfected with NCAPH expression vector and control vector were determined by qRT‐PCR and Western blot. (c,d) The mRNA and protein levels of NCAPH in UMUC3 cells transfected with NCAPH siRNA1-3 and negative control (siNC) were determined by qRT‐PCR and Western blot. (e-h) The effect of NCAPH on cell proliferation of BC cells was determined by BrdU assay (Scale bar, 100 μm) and MTT assay. The data were presented as the mean ± SD.

Application: IF/ICC    Species: Mouse    Sample: SW780 cells

Figure 2. NCAPH promotes the cell proliferation of BC cells. (a,b) The mRNA and protein levels of NCAPH in SW780 cells transfected with NCAPH expression vector and control vector were determined by qRT‐PCR and Western blot. (c,d) The mRNA and protein levels of NCAPH in UMUC3 cells transfected with NCAPH siRNA1-3 and negative control (siNC) were determined by qRT‐PCR and Western blot. (e-h) The effect of NCAPH on cell proliferation of BC cells was determined by BrdU assay (Scale bar, 100 μm) and MTT assay. The data were presented as the mean ± SD.

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