Product: GFM2 Antibody
Catalog: DF4035
Description: Rabbit polyclonal antibody to GFM2
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Horse, Dog
Mol.Wt.: 87 KD; 87kD(Calculated).
Uniprot: Q969S9
RRID: AB_2836328

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Horse(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
GFM2 Antibody detects endogenous levels of total GFM2.
RRID:
AB_2836328
Cite Format: Affinity Biosciences Cat# DF4035, RRID:AB_2836328.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EF-G2mt; EFG2; EFG2mt; elongation factor G 2, mitochondrial; Elongation factor G2; G elongation factor mitochondrial 2; GFM2; hEFG2; mEFG 2; mEFG2; Mitochondrial elongation factor G2; mitochondrial ribosome recycling factor 2; MRRF2; MST027; MSTP027; OTTHUMP00000222951; OTTHUMP00000222952; ribosome-releasing factor 2, mitochondrial; RRF2; RRF2mt;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q969S9 RRF2M_HUMAN:

Widely expressed.

Sequence:
MLTNLRIFAMSHQTIPSVYINNICCYKIRASLKRLKPHVPLGRNCSSLPGLIGNDIKSLHSIINPPIAKIRNIGIMAHIDAGKTTTTERILYYSGYTRSLGDVDDGDTVTDFMAQERERGITIQSAAVTFDWKGYRVNLIDTPGHVDFTLEVERCLRVLDGAVAVFDASAGVEAQTLTVWRQADKHNIPRICFLNKMDKTGASFKYAVESIREKLKAKPLLLQLPIGEAKTFKGVVDVVMKEKLLWNCNSNDGKDFERKPLLEMNDPELLKETTEARNALIEQVADLDDEFADLVLEEFSENFDLLPAEKLQTAIHRVTLAQTAVPVLCGSALKNKGIQPLLDAVTMYLPSPEERNYEFLQWYKDDLCALAFKVLHDKQRGPLVFMRIYSGTIKPQLAIHNINGNCTERISRLLLPFADQHVEIPSLTAGNIALTVGLKHTATGDTIVSSKSSALAAARRAEREGEKKHRQNNEAERLLLAGVEIPEPVFFCTIEPPSLSKQPDLEHALKCLQREDPSLKVRLDPDSGQTVLCGMGELHIEIIHDRIKREYGLETYLGPLQVAYRETILNSVRATDTLDRTLGDKRHLVTVEVEARPIETSSVMPVIEFEYAESINEGLLKVSQEAIENGIHSACLQGPLLGSPIQDVAITLHSLTIHPGTSTTMISACVSRCVQKALKKADKQVLEPLMNLEVTVARDYLSPVLADLAQRRGNIQEIQTRQDNKVVIGFVPLAEIMGYSTVLRTLTSGSATFALELSTYQAMNPQDQNTLLNRRSGLT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Dog
91
Horse
82
Bovine
73
Chicken
57
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969S9 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
T14 Phosphorylation
S31 Phosphorylation
K196 Ubiquitination
K199 Ubiquitination
K205 Ubiquitination
K218 Ubiquitination
K230 Ubiquitination
K233 Acetylation
K243 Ubiquitination
K259 Ubiquitination
K271 Acetylation
Y348 Phosphorylation
K373 Ubiquitination
K394 Ubiquitination
S426 Phosphorylation
K451 Ubiquitination
K510 Ubiquitination
K520 Ubiquitination
Y556 Phosphorylation
Y564 Phosphorylation
Y611 Phosphorylation
S614 Phosphorylation
Y700 Phosphorylation
S702 Phosphorylation

Research Backgrounds

Function:

Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.

Restrictive clause

 

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