DKK3 Antibody | Affinity Biosciences

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DKK3 Antibody - Western blot analysis of extracts from Jurkat cells, using DKK3 antibody.

Product:	DKK3 Antibody
Catalog:	DF3990
Description:	Rabbit polyclonal antibody to DKK3
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	45 KD; 38kD(Calculated).
Uniprot:	Q9UBP4
RRID:	AB_2836350

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

DKK3 Antibody detects endogenous levels of total DKK3.

RRID:

AB_2836350
Cite Format: Affinity Biosciences Cat# DF3990, RRID:AB_2836350.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dickkopf 3; Dickkopf 3 homolog (Xenopus laevis); Dickkopf 3 homolog; Dickkopf homolog 3; Dickkopf related protein 3; Dickkopf related protein 3 Precursor; Dickkopf WNT signaling pathway inhibitor 3; Dickkopf, Xenopus, homolog of, 3; Dickkopf-3; Dickkopf-related protein 3; Dickkopf3; DKK 3; Dkk-3; DKK3; DKK3_HUMAN; hDkk 3; hDkk-3; hDkk3; Regulated in glioma; REIC; RIG; RIG-like 5-6; RIG-like 7-1;

Immunogens

Immunogen:

Uniprot:

Q9UBP4(DKK3_HUMAN) >>Visit HPA database.

Gene(ID):

DKK3(27122)

Expression:

Q9UBP4 DKK3_HUMAN:

Highest expression in heart, brain, and spinal cord.

Sequence:

Q9UBP4 DKK3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQRLGATLLCLLLAAAVPTAPAPAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALREPAAAAAALLGGEEI

PTMs - Q9UBP4 As Substrate

Q9UBP4

Site	PTM Type	Source
T26	O-Glycosylation	Uniprot
T28	O-Glycosylation	Uniprot
T119	Phosphorylation	Uniprot
S129	Phosphorylation	Uniprot

Research Backgrounds

Q9UBP4_DKK3_HUMAN

Function:

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

PTMs:

N- and O-glycosylated.

Subcellular Location:

Secreted.

Tissue Specificity:

Highest expression in heart, brain, and spinal cord.

Subunit Structure:

Interacts with LRP5 and LRP6.

Family&Domains:

The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

Belongs to the dickkopf family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

DKK3 Antibody - #DF3990