RNF111 Antibody - #DF4015
| Product: | RNF111 Antibody |
| Catalog: | DF4015 |
| Description: | Rabbit polyclonal antibody to RNF111 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 109 KD; 109kD(Calculated). |
| Uniprot: | Q6ZNA4 |
| RRID: | AB_2836375 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4015, RRID:AB_2836375.
Fold/Unfold
ARK; Arkadia; DKFZp313E0731; DKFZp686H1966; DKFZp761D081; E3 ubiquitin protein ligase Arkadia; E3 ubiquitin-protein ligase Arkadia; FLJ38008; RING finger protein 111; RN111_HUMAN; RNF 111; RNF111;
Immunogens
- Q6ZNA4 RN111_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQWTPEYNELYTLKVDMKSEIPSDAPKTQESLKGILLHPEPIGAAKSFPAGVEMINSKVGNEFSHLCDDSQKQEKEMNGNQQEQEKSLVVRKKRKSQQAGPSYVQNCVKENQGILGLRQHLGTPSDEDNDSSFSDCLSSPSSSLHFGDSDTVTSDEDKEVSVRHSQTILNAKSRSHSARSHKWPRTETESVSGLLMKRPCLHGSSLRRLPCRKRFVKNNSSQRTQKQKERILMQRKKREVLARRKYALLPSSSSSSENDLSSESSSSSSTEGEEDLFVSASENHQNNPAVPSGSIDEDVVVIEASSTPQVTANEEINVTSTDSEVEIVTVGESYRSRSTLGHSRSHWSQGSSSHASRPQEPRNRSRISTVIQPLRQNAAEVVDLTVDEDEPTVVPTTSARMESQATSASINNSNPSTSEQASDTASAVTSSQPSTVSETSATLTSNSTTGTSIGDDSRRTTSSAVTETGPPAMPRLPSCCPQHSPCGGSSQNHHALGHPHTSCFQQHGHHFQHHHHHHHTPHPAVPVSPSFSDPACPVERPPQVQAPCGANSSSGTSYHEQQALPVDLSNSGIRSHGSGSFHGASAFDPCCPVSSSRAAIFGHQAAAAAPSQPLSSIDGYGSSMVAQPQPQPPPQPSLSSCRHYMPPPYASLTRPLHHQASACPHSHGNPPPQTQPPPQVDYVIPHPVHAFHSQISSHATSHPVAPPPPTHLASTAAPIPQHLPPTHQPISHHIPATAPPAQRLHPHEVMQRMEVQRRRMMQHPTRAHERPPPHPHRMHPNYGHGHHIHVPQTMSSHPRQAPERSAWELGIEAGVTAATYTPGALHPHLAHYHAPPRLHHLQLGALPLMVPDMAGYPHIRYISSGLDGTSFRGPFRGNFEELIHLEERLGNVNRGASQGTIERCTYPHKYKKVTTDWFSQRKLHCKQDGEEGTEEDTEEKCTICLSILEEGEDVRRLPCMHLFHQVCVDQWLITNKKCPICRVDIEAQLPSES
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6ZNA4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K19 | Sumoylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| K34 | Sumoylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K47 | Sumoylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K73 | Sumoylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| K173 | Sumoylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| Y783 | Phosphorylation | Uniprot | |
| R861 | Methylation | Uniprot | |
| R895 | Methylation | Uniprot | |
| S898 | Phosphorylation | Uniprot | |
| K927 | Sumoylation | Uniprot | |
| K927 | Ubiquitination | Uniprot |
Research Backgrounds
E3 ubiquitin-protein ligase. Required for mesoderm patterning during embryonic development (By similarity). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP. Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP. In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL. Associates with UBE2D2 as an E2 enzyme. Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates. Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair. Mediates ubiquitination and degradation of sumoylated PML (By similarity). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (By similarity).
Nucleus. Cytoplasm. Nucleus>PML body.
Note: Upon TGF-beta treatment, translocates from nucleus to cytosol.
Broadly expressed.
Monomer. Interacts with SMAD6, SMAD7, AXIN1, AXIN2 and SKIL isoform SNON. Interacts with (phosphorylated) SMAD2 and SMAD3 (By similarity). Part of a complex containing RNF111, AXIN1 and SMAD7. Interacts (via SIM domains) with SUMO1 and SUMO2.
The SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate.
The RING-type zinc finger mediates the E3 ubiquitin-protein ligase activity and binds directly to free ubiquitin (PubMed:26656854). Non-covalent ubiquitin-binding stabilizes the ubiquitin-conjugating enzyme E2 (donor ubiquitin) in the 'closed' conformation and stimulates ubiquitin transfer (By similarity).
Belongs to the Arkadia family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.