PJA2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

PJA2 Antibody - Western blot analysis of extracts from VERO cells, using PJA2 Antibody.

PJA2 Antibody - DF4021 staining Hela cells by IF/ICC.

Product:	PJA2 Antibody
Catalog:	DF4021
Description:	Rabbit polyclonal antibody to PJA2
Application:	WB IF/ICC
Reactivity:	Human, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	85 KD; 78kD(Calculated).
Uniprot:	O43164
RRID:	AB_2836381

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Pig(91%), Bovine(90%), Horse(91%), Sheep(80%), Rabbit(82%)

Clonality:

Polyclonal

Specificity:

PJA2 Antibody detects endogenous levels of total PJA2.

RRID:

AB_2836381
Cite Format: Affinity Biosciences Cat# DF4021, RRID:AB_2836381.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

E3 ubiquitin protein ligase Praja2; E3 ubiquitin-protein ligase Praja-2; KIAA0438; Neurodap 1; Neurodap1; PJA 2; PJA2; PJA2_HUMAN; Praja 2; Praja 2 RING H2 motif containing; praja ring finger 2; Praja ring finger 2 E3 ubiquitin protein ligase; Praja2; RING finger protein 131; RNF 131; RNF131;

Immunogens

Immunogen:

Uniprot:

O43164(PJA2_HUMAN) >>Visit HPA database.

Gene(ID):

PJA2(9867)

Sequence:

O43164 PJA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTEHSPEDAACGPGHICSEQNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGATAGRQEVDNTFWNGCGDYYQLYDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIEASAAPSSEPDPDAPPSNDSIAEAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Rabbit

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43164 As Substrate

O43164

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
Y4	Phosphorylation		Uniprot
K18	Ubiquitination		Uniprot
Y28	Phosphorylation		Uniprot
T30	Phosphorylation		Uniprot
Y42	Phosphorylation		Uniprot
S44	Phosphorylation		Uniprot
K46	Acetylation		Uniprot
K46	Methylation		Uniprot
Y63	Phosphorylation		Uniprot
S76	Phosphorylation		Uniprot
S80	Phosphorylation		Uniprot
S134	Phosphorylation		Uniprot
S167	Phosphorylation		Uniprot
Y168	Phosphorylation		Uniprot
Y193	Phosphorylation		Uniprot
S196	Phosphorylation		Uniprot
Y212	Phosphorylation		Uniprot
T213	Phosphorylation		Uniprot
S216	Phosphorylation		Uniprot
S235	Phosphorylation		Uniprot
T246	Phosphorylation		Uniprot
S253	Phosphorylation		Uniprot
K268	Ubiquitination		Uniprot
S282	Phosphorylation		Uniprot
K304	Ubiquitination		Uniprot
S308	Phosphorylation		Uniprot
S309	Phosphorylation		Uniprot
K317	Ubiquitination		Uniprot
K320	Ubiquitination		Uniprot
S323	Phosphorylation		Uniprot
S324	Phosphorylation		Uniprot
K339	Ubiquitination		Uniprot
S342	Phosphorylation	P17612 (PRKACA)	Uniprot
Y367	Phosphorylation		Uniprot
Y380	Phosphorylation		Uniprot
S381	Phosphorylation		Uniprot
T385	Phosphorylation		Uniprot
T389	Phosphorylation	P17612 (PRKACA)	Uniprot
S396	Phosphorylation		Uniprot
S428	Phosphorylation		Uniprot
S429	Phosphorylation		Uniprot
S432	Phosphorylation		Uniprot
S452	Phosphorylation		Uniprot
S453	Phosphorylation		Uniprot
S454	Phosphorylation		Uniprot
S457	Phosphorylation		Uniprot
T460	Phosphorylation		Uniprot
S596	Phosphorylation		Uniprot
K658	Ubiquitination		Uniprot
K667	Ubiquitination		Uniprot

Research Backgrounds

O43164_PJA2_HUMAN

Function:

Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes. Through the ubiquitination of MFHAS1, positively regulates the TLR2 signaling pathway that leads to the activation of the downstream p38 and JNK MAP kinases and promotes the polarization of macrophages toward the pro-inflammatory M1 phenotype.

Subcellular Location:

Cytoplasm. Cell membrane. Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein. Cell junction>Synapse. Cell junction>Synapse>Postsynaptic density.
Note: Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses (By similarity). Colocalizes with PRKAR2A and PRKAR2B in the cytoplasm and the cell membrane.

Subunit Structure:

Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. The phosphorylated form interacts with PRKAR1A, PRKAR2A and PRKAR2B. Binds the catalytic subunits of cAMP-dependent protein kinase. Interacts with MFHAS1.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PJA2 Antibody - #DF4021