MYO5A Antibody - #DF4200

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Product: MYO5A Antibody
Catalog: DF4200
Description: Rabbit polyclonal antibody to MYO5A
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 220 KD; 215kD(Calculated).
Uniprot: Q9Y4I1
RRID: AB_2836409

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
MYO5A Antibody detects endogenous levels of total MYO5A.
RRID:
AB_2836409
Cite Format: Affinity Biosciences Cat# DF4200, RRID:AB_2836409.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

non-muscle; Dilute myosin heavy chain; GS1; MYH12; MYO5; Myo5a; MYO5A_HUMAN; Myosin heavy chain 12; Myosin heavy polypeptide kinase; Myosin V; Myosin VA (heavy polypeptide 12 myoxin); Myosin VA (heavy polypeptide 12 myoxin); Myosin-12; Myoxin; MYR12; Unconventional myosin-Va;

Immunogens

Immunogen:
Uniprot:

Q9Y4I1(MYO5A_HUMAN) >>Visit HPA database.

Gene(ID):
MYO5A(4644)
Expression:
Q9Y4I1 MYO5A_HUMAN:

Detected in melanocytes.

Sequence:
MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLGNADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTIPPKHEPLCIFCELMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVNQALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQCEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAATIVLQSYLRGFLARNRYRKILREHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKIEARSVERYKKLHIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAKEMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSNESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKEEERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELDVRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4I1 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K9 Ubiquitination
Y31 Phosphorylation
K169 Ubiquitination
S172 Phosphorylation
Y175 Phosphorylation
S199 Phosphorylation
S205 Phosphorylation
K246 Ubiquitination
K289 Ubiquitination
S600 Phosphorylation
T610 Phosphorylation
T615 Phosphorylation
S721 Phosphorylation
Y757 Phosphorylation
Y828 Phosphorylation
Y834 Phosphorylation
K902 Acetylation
Y918 Phosphorylation
K979 Ubiquitination
S987 Phosphorylation
K1028 Ubiquitination
T1032 Phosphorylation
K1050 Methylation
K1058 Ubiquitination
Y1078 Phosphorylation
S1086 Phosphorylation
T1113 Phosphorylation
S1115 Phosphorylation
T1116 Phosphorylation
S1118 Phosphorylation
S1119 Phosphorylation
Y1203 Phosphorylation
S1205 Phosphorylation
K1207 Acetylation
K1207 Ubiquitination
S1213 Phosphorylation
K1232 Ubiquitination
Y1312 Phosphorylation
Y1339 Phosphorylation
S1400 Phosphorylation
T1409 Phosphorylation
S1452 Phosphorylation
Y1511 Phosphorylation
S1531 Phosphorylation
T1534 Phosphorylation
S1535 Phosphorylation
T1536 Phosphorylation
S1539 Phosphorylation
S1572 Phosphorylation
T1650 Phosphorylation P31751 (AKT2)
S1651 Phosphorylation
S1652 Phosphorylation
T1658 Phosphorylation
T1743 Phosphorylation

Research Backgrounds

Function:

Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.

Tissue Specificity:

Detected in melanocytes.

Subunit Structure:

May be a homodimer, which associates with multiple calmodulin or myosin light chains (By similarity). Interacts with RIPL2, the interaction is required for its role in dendrite formation (By similarity). Interacts with MLPH. Interacts with SYTL4 (By similarity). Interacts with MYRIP (By similarity). Interacts with RAB10; mediates the transport to the plasma membrane of SLC2A4/GLUT4 storage vesicles. Interacts with FMR1; this interaction occurs in association with polyribosome (By similarity).

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

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