Myoglobin Antibody - #BF0424
(1)
| Product: | Myoglobin Antibody |
| Catalog: | BF0424 |
| Description: | Mouse monoclonal antibody to Myoglobin |
| Application: | WB IHC ELISA |
| Reactivity: | Human |
| Mol.Wt.: | 17kDa.; 17kD(Calculated). |
| Uniprot: | P02144 |
| RRID: | AB_2833582 |
Product Info
Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Monoclonal [AFB1557]
Specificity:
Myoglobin antibody detects endogenous levels of total Myoglobin.
RRID:
AB_2833582
Cite Format: Affinity Biosciences Cat# BF0424, RRID:AB_2833582.
Cite Format: Affinity Biosciences Cat# BF0424, RRID:AB_2833582.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
MB; MGC13548; MYG_HUMAN; Myoglobin; PVALB;
Immunogens
Immunogen:
Purified recombinant fragment of human Myoglobin expressed in E. Coli.
Uniprot:
Gene(ID):
Description:
Myoglobin (MB), with 154-amino acid protein (about 17kDa), is a member of the globin superfamily and expression of myoglobin is highest in skeletal and cardiac muscle. Functionally, myoglobin is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O2 concentration when muscle activity increases and to facilitate intracellular O2 diffusion by providing a parallel path that augments simple diffusion of dissolved O2. Furthermore, myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.
Sequence:
- P02144 MYG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG
PTMs - P02144 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K17 | Acetylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K57 | Acetylation | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K64 | Acetylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| Y147 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Family&Domains:
Belongs to the globin family.
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For Research Use Only.
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