GCF Antibody - #DF4083
| Product: | GCF Antibody |
| Catalog: | DF4083 |
| Description: | Rabbit polyclonal antibody to GCF |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Bovine, Horse, Sheep |
| Mol.Wt.: | 80 KD; 89kD(Calculated). |
| Uniprot: | P16383 |
| RRID: | AB_2836457 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4083, RRID:AB_2836457.
Fold/Unfold
Chromosome 2 open reading frame 3; DNABF; GC rich sequence DNA binding factor; GCF; TCF 9; TCF9; Transcription factor 9;
Immunogens
- P16383 GCFC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAHRPKRTFRQRAADSSDSDGAEESPAEPGAPRELPVPGSAEEEPPSGGGRAQVAGLPHRVRGPRGRGRVWASSRRATKAAPRADEGSESRTLDVSTDEEDKIHHSSESKDDQGLSSDSSSSLGEKELSSTVKIPDAAFIQAARRKRELARAQDDYISLDVQHTSSISGMKRESEDDPESEPDDHEKRIPFTLRPQTLRQRMAEESISRNEETSEESQEDEKQDTWEQQQMRKAVKIIEERDIDLSCGNGSSKVKKFDTSISFPPVNLEIIKKQLNTRLTLLQETHRSHLREYEKYVQDVKSSKSTIQNLESSSNQALNCKFYKSMKIYVENLIDCLNEKIINIQEIESSMHALLLKQAMTFMKRRQDELKHESTYLQQLSRKDETSTSGNFSVDEKTQWILEEIESRRTKRRQARVLSGNCNHQEGTSSDDELPSAEMIDFQKSQGDILQKQKKVFEEVQDDFCNIQNILLKFQQWREKFPDSYYEAFISLCIPKLLNPLIRVQLIDWNPLKLESTGLKEMPWFKSVEEFMDSSVEDSKKESSSDKKVLSAIINKTIIPRLTDFVEFLWDPLSTSQTTSLITHCRVILEEHSTCENEVSKSRQDLLKSIVSRMKKAVEDDVFIPLYPKSAVENKTSPHSKFQERQFWSGLKLFRNILLWNGLLTDDTLQELGLGKLLNRYLIIALLNATPGPDVVKKCNQVAACLPEKWFENSAMRTSIPQLENFIQFLLQSAHKLSRSEFRDEVEEIILILVKIKALNQAESFIGEHHLDHLKSLIKED
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P16383 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| R51 | Methylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| K79 | Methylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K411 | Acetylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| T428 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| T557 | Phosphorylation | Uniprot | |
| Y627 | Phosphorylation | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| S637 | Phosphorylation | Uniprot | |
| Y681 | Phosphorylation | Uniprot | |
| K775 | Ubiquitination | Uniprot |
Research Backgrounds
Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. Involved in pre-mRNA splicing through regulating spliceosome C complex formation. May play a role during late-stage splicing events and turnover of excised inrons.
Nucleus>Nucleoplasm. Nucleus>Nucleolus.
Widely expressed in tissues and cell lines.
Found in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors. Interacts with TFIP11 and DHX15.
Belongs to the GCF family.
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