GRB14 Antibody - #DF4091
| Product: | GRB14 Antibody |
| Catalog: | DF4091 |
| Description: | Rabbit polyclonal antibody to GRB14 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 61 KD; 61kD(Calculated). |
| Uniprot: | Q14449 |
| RRID: | AB_2836465 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4091, RRID:AB_2836465.
Fold/Unfold
GRB14 adapter protein; Grb14; GRB14_HUMAN; Growth factor receptor bound protein 14;
Immunogens
Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.
- Q14449 GRB14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSLQDGQSAASRAAARDSPLAAQVCGAAQGRGDAHDLAPAPWLHARALLPLPDGTRGCAADRRKKKDLDVPEMPSIPNPFPELCCSPFTSVLSADLFPKANSRKKQVIKVYSEDETSRALDVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHIGVERTIEDHELVIEVLSNWGIEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFFLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGCSSQSISPMRSISENSLVAMDFSGQKSRVIENPTEALSVAVEEGLAWRKKGCLRLGTHGSPTASSQSSATNMAIHRSQPWFHHKISRDEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14449 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| Y347 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| T421 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot |
Research Backgrounds
Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2.
Cytoplasm. Endosome membrane>Peripheral membrane protein.
Note: Upon insulin stimulation, translocates to the plasma membrane.
Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.
Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor (INSR), through the SH2 domain (By similarity). Interacts with GRB14 (via BPS domain); this interaction protects the tyrosines in the activation loop on INSR from dephosphorylation. Binds to the ankyrin repeat region of TNKS2 via its N-terminus. Interacts with activated NRAS. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated).
The PH domain binds relatively non-specifically and with low affinity to several phosphoinositides, the best binder being PI(3,4,5)P3.
Belongs to the GRB7/10/14 family.
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