SIX5 Antibody - #DF4130
| Product: | SIX5 Antibody |
| Catalog: | DF4130 |
| Description: | Rabbit polyclonal antibody to SIX5 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Bovine, Sheep |
| Mol.Wt.: | 75 KD; 75kD(Calculated). |
| Uniprot: | Q8N196 |
| RRID: | AB_2836495 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4130, RRID:AB_2836495.
Fold/Unfold
BOR2; DM locus associated homeodomain protein; DM locus-associated homeodomain protein; DMAHP; Dystrophia myotonica associated homeodomain protein; Homeobox protein SIX5; Sine oculis homeobox homolog 5; sine oculis related homeobox 5 homolog (Drosophila); SIX homeobox 5; SIX5; SIX5_HUMAN;
Immunogens
Expressed in adult but not in fetal eyes. Found in corneal epithelium and endothelium, lens epithelium, ciliary body epithelia, cellular layers of the retina and the sclera.
- Q8N196 SIX5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATLPAEPSAGPAAGGEAVAAAAATEEEEEEARQLLQTLQAAEGEAAAAAGAGAGAAAAGAEGPGSPGVPGSPPEAASEPPTGLRFSPEQVACVCEALLQAGHAGRLSRFLGALPPAERLRGSDPVLRARALVAFQRGEYAELYRLLESRPFPAAHHAFLQDLYLRARYHEAERARGRALGAVDKYRLRKKFPLPKTIWDGEETVYCFKERSRAALKACYRGNRYPTPDEKRRLATLTGLSLTQVSNWFKNRRQRDRTGAGGGAPCKSESDGNPTTEDESSRSPEDLERGAAPVSAEAAAQGSIFLAGTGPPAPCPASSSILVNGSFLAASGSPAVLLNGGPVIINGLALGEASSLGPLLLTGGGGAPPPQPSPQGASETKTSLVLDPQTGEVRLEEAQSEAPETKGAQVAAPGPALGEEVLGPLAQVVPGPPTAATFPLPPGPVPAVAAPQVVPLSPPPGYPTGLSPTSPLLNLPQVVPTSQVVTLPQAVGPLQLLAAGPGSPVKVAAAAGPANVHLINSGVGVTALQLPSATAPGNFLLANPVSGSPIVTGVALQQGKIILTATFPTSMLVSQVLPPAPGLALPLKPETAISVPEGGLPVAPSPALPEAHALGTLSAQQPPPAAATTSSTSLPFSPDSPGLLPNFPAPPPEGLMLSPAAVPVWSAGLELSAGTEGLLEAEKGLGTQAPHTVLRLPDPDPEGLLLGATAGGEVDEGLEAEAKVLTQLQSVPVEEPLEL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8N196 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S66 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| R121 | Methylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| R168 | Methylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| T275 | Phosphorylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| T380 | Phosphorylation | Uniprot |
Research Backgrounds
Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter (By similarity). Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3 (By similarity).
Cytoplasm. Nucleus.
Expressed in adult but not in fetal eyes. Found in corneal epithelium and endothelium, lens epithelium, ciliary body epithelia, cellular layers of the retina and the sclera.
Probably binds DNA dimer. Interacts with EYA3, and probably EYA1 and EYA2 (By similarity).
Belongs to the SIX/Sine oculis homeobox family.
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