IQCB1 Antibody - #DF4133

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Product: IQCB1 Antibody
Catalog: DF4133
Description: Rabbit polyclonal antibody to IQCB1
Application: WB IF/ICC
Reactivity: Human
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 69 KD; 69kD(Calculated).
Uniprot: Q15051
RRID: AB_2836498

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(82%), Rabbit(91%), Dog(82%)
Clonality:
Polyclonal
Specificity:
IQCB1 Antibody detects endogenous levels of total IQCB1.
RRID:
AB_2836498
Cite Format: Affinity Biosciences Cat# DF4133, RRID:AB_2836498.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IQ calmodulin-binding motif containing 1; IQ calmodulin-binding motif-containing protein 1; IQ motif containing B1; Iqcb1; IQCB1_HUMAN; KIAA0036; Nephrocystin-5; NPHP5; p53 and DNA damage-regulated IQ motif protein; SLSN5;

Immunogens

Immunogen:
Uniprot:

Q15051(IQCB1_HUMAN) >>Visit HPA database.

Gene(ID):
IQCB1(9657)
Expression:
Q15051 IQCB1_HUMAN:

Ubiquitously expressed in fetal and adult tissues. Localized to the outer segments and connecting cilia of photoreceptor cells. Up-regulated in a number of primary colorectal and gastric tumors.

Sequence:
MKPTGTDPRILSIAAEVAKSPEQNVPVILLKLKEIINITPLGSSELKKIKQDIYCYDLIQYCLLVLSQDYSRIQGGWTTISQLTQILSHCCVGLEPGEDAEEFYNELLPSAAENFLVLGRQLQTCFINAAKAEEKDELLHFFQIVTDSLFWLLGGHVELIQNVLQSDHFLHLLQADNVQIGSAVMMMLQNILQINSGDLLRIGRKALYSILDEVIFKLFSTPSPVIRSTATKLLLLMAESHQEILILLRQSTCYKGLRRLLSKQETGTEFSQELRQLVGLLSPMVYQEVEEQKLHQAACLIQAYWKGFQTRKRLKKLPSAVIALQRSFRSKRSKMLLEINRQKEEEDLKLQLQLQRQRAMRLSRELQLSMLEIVHPGQVEKHYREMEEKSALIIQKHWRGYRERKNFHQQRQSLIEYKAAVTLQRAALKFLAKCRKKKKLFAPWRGLQELTDARRVELKKRVDDYVRRHLGSPMSDVVSRELHAQAQERLQHYFMGRALEERAQQHREALIAQISTNVEQLMKAPSLKEAEGKEPELFLSRSRPVAAKAKQAHLTTLKHIQAPWWKKLGEESGDEIDVPKDELSIELENLFIGGTKPP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Horse
82
Dog
82
Pig
73
Bovine
73
Sheep
73
Zebrafish
63
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15051 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
S12 Phosphorylation
K19 Ubiquitination
S20 Phosphorylation
K31 Ubiquitination
K33 Ubiquitination
K47 Ubiquitination
K205 Ubiquitination
K263 Ubiquitination
R326 Methylation
S327 Phosphorylation
K331 Methylation
S333 Phosphorylation
K334 Ubiquitination
S363 Phosphorylation
S369 Phosphorylation
K396 Ubiquitination
S472 Phosphorylation
K528 Ubiquitination
R541 Methylation
K550 Ubiquitination
K566 Ubiquitination
S572 Phosphorylation

Research Backgrounds

Function:

Involved in ciliogenesis. The function in an early step in cilia formation depends on its association with CEP290/NPHP6. Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2 and BBS5 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating CEP290/NPHP6.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: Localization to the centrosome depends on the interaction with CEP290/NPHP6.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in fetal and adult tissues. Localized to the outer segments and connecting cilia of photoreceptor cells. Up-regulated in a number of primary colorectal and gastric tumors.

Subunit Structure:

Interacts with CEP290/NPHP6; IQCB1/NPHP5 and CEP290 are proposed to form a functional NPHP5-6 module/NPHP6; localized to the centrosome. Interacts with calmodulin, ATXN10. Interacts with NPHP1, INVS, NPHP4 and RPGRIP1L; these interactions likely require additional interactors (By similarity). Associates with the BBSome complex; interacts with BBS1, BBS2, BBS4, BBS5, BBS7, BBS8 and BBS9.

Family&Domains:

The IQ domains mediate the interaction with calmodulin.

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