MAP9 Antibody | Affinity Biosciences

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MAP9 Antibody - Western blot analysis of extracts from PC12 cells(H2O2 treatment), using MAP9 Antibody.

Product:	MAP9 Antibody
Catalog:	DF4163
Description:	Rabbit polyclonal antibody to MAP9
Application:	WB IF/ICC
Reactivity:	Human, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	74 KD; 74kD(Calculated).
Uniprot:	Q49MG5
RRID:	AB_2836528

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

MAP9 Antibody detects endogenous levels of total MAP9.

RRID:

AB_2836528
Cite Format: Affinity Biosciences Cat# DF4163, RRID:AB_2836528.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ASAP; Aster associated protein; Aster-associated protein; Map9; MAP9_HUMAN; microtubule associated protein 9; Microtubule-associated protein 9;

Immunogens

Immunogen:

Uniprot:

Q49MG5(MAP9_HUMAN) >>Visit HPA database.

Gene(ID):

MAP9(79884)

Sequence:

Q49MG5 MAP9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSDEVFSTTLAYTKSPKVTKRTTFQDELIRAITARSARQRSSEYSDDFDSDEIVSLGDFSDTSADENSVNKKMNDFHISDDEEKNPSKLLFLKTNKSNGNITKDEPVCAIKNEEEMAPDGCEDIVVKSFSESQNKDEEFEKDKIKMKPKPRILSIKSTSSAENNSLDTDDHFKPSPRPRSMLKKKSHMEEKDGLEDKETALSEELELHSAPSSLPTPNGIQLEAEKKAFSENLDPEDSCLTSLASSSLKQILGDSFSPGSEGNASGKDPNEEITENHNSLKSDENKENSFSADHVTTAVEKSKESQVTADDLEEEKAKAELIMDDDRTVDPLLSKSQSILISTSATASSKKTIEDRNIKNKKSTNNRASSASARLMTSEFLKKSSSKRRTPSTTTSSHYLGTLKVLDQKPSQKQSIEPDRADNIRAAVYQEWLEKKNVYLHEMHRIKRIESENLRIQNEQKKAAKREEALASFEAWKAMKEKEAKKIAAKKRLEEKNKKKTEEENAARKGEALQAFEKWKEKKMEYLKEKNRKEREYERAKKQKEEETVAEKKKDNLTAVEKWNEKKEAFFKQKEKEKINEKRKEELKRAEKKDKDKQAINEYEKWLENKEKQERIERKQKKRHSFLESEALPPWSPPSRTVFAKVF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Xenopus

Pig

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q49MG5 As Substrate

Q49MG5

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
Y12	Phosphorylation		Uniprot
S15	Phosphorylation		Uniprot
S41	Phosphorylation		Uniprot
S79	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
S130	Phosphorylation		Uniprot
S132	Phosphorylation		Uniprot
S175	Phosphorylation		Uniprot
S180	Phosphorylation		Uniprot
S257	Phosphorylation		Uniprot
S265	Phosphorylation		Uniprot
S289	Phosphorylation	P53350 (PLK1)	Uniprot
S291	Phosphorylation		Uniprot
S305	Phosphorylation	O14965 (AURKA)	Uniprot
S336	Phosphorylation		Uniprot
T343	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
S385	Phosphorylation		Uniprot
T390	Phosphorylation		Uniprot
S392	Phosphorylation		Uniprot
T393	Phosphorylation		Uniprot
T394	Phosphorylation		Uniprot
S396	Phosphorylation		Uniprot
S397	Phosphorylation		Uniprot
Y429	Phosphorylation		Uniprot
K496	Acetylation		Uniprot
Y603	Phosphorylation		Uniprot
S625	Phosphorylation	O14965 (AURKA)	Uniprot
S636	Phosphorylation		Uniprot

Research Backgrounds

Q49MG5_MAP9_HUMAN

Function:

Involved in organization of the bipolar mitotic spindle. Required for bipolar spindle assembly, mitosis progression and cytokinesis. May act by stabilizing interphase microtubules.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle.
Note: Localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis.

Subunit Structure:

Binds to purified microtubules via its C-terminus.

Restrictive clause

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For Research Use Only.
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MAP9 Antibody - #DF4163