MRPS27 Antibody | Affinity Biosciences

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Product:	MRPS27 Antibody
Catalog:	DF4168
Description:	Rabbit polyclonal antibody to MRPS27
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	48 KD; 48kD(Calculated).
Uniprot:	Q92552
RRID:	AB_2836533

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Bovine(82%), Horse(91%), Sheep(91%), Rabbit(82%), Dog(91%)

Clonality:

Polyclonal

Specificity:

MRPS27 Antibody detects endogenous levels of total MRPS27.

RRID:

AB_2836533
Cite Format: Affinity Biosciences Cat# DF4168, RRID:AB_2836533.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

28S ribosomal protein S27; 28S ribosomal protein S27, mitochondrial; KIAA0264; Mitochondrial 28S ribosomal protein S27; mitochondrial; mitochondrial ribosomal protein S27; MRP-S27; MRPS27; RT27_HUMAN; S27mt;

Immunogens

Immunogen:

A synthesized peptide derived from human MRPS27, corresponding to a region within N-terminal amino acids.

Uniprot:

Q92552(RT27_HUMAN) >>Visit HPA database.

Gene(ID):

MRPS27(23107)

Expression:

Q92552 RT27_HUMAN:

Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues (at protein level) (PubMed:28714366). Ubiquitous (PubMed:22841715).

Sequence:

Q92552 RT27_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEAREKEHYCLADLASLMDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKFRHSPNCWYLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDALSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNSVGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Sheep

Dog

Bovine

Rabbit

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q92552_RT27_HUMAN

Function:

RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis. Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain. Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu). Involved also in positive regulation of cell proliferation and tumor cell growth.

Subcellular Location:

Cytoplasm. Mitochondrion.

Tissue Specificity:

Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues (at protein level). Ubiquitous.

Family&Domains:

Belongs to the mitochondrion-specific ribosomal protein mS27 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MRPS27 Antibody - #DF4168