Product: MRPS30 Antibody
Catalog: DF4169
Description: Rabbit polyclonal antibody to MRPS30
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 55 KD; 50kD(Calculated).
Uniprot: Q9NP92
RRID: AB_2836534

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
MRPS30 Antibody detects endogenous levels of total MRPS30.
RRID:
AB_2836534
Cite Format: Affinity Biosciences Cat# DF4169, RRID:AB_2836534.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

28S ribosomal protein S30; 28S ribosomal protein S30, mitochondrial; Mitochondrial 28S ribosomal protein S30; mitochondrial; Mitochondrial ribosomal protein S30; MRP S30; MRP-S30; MRPS30; PAP; PDCD9; Programmed cell death 9; Programmed cell death protein 9; RT30_HUMAN; S30mt;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9NP92 RT30_HUMAN:

Heart, skeletal muscle, kidney and liver. Lower expression in placenta and peripheral blood leukocytes.

Sequence:
MAAARCWRPLLRGPRLSLHTAANAAATATETTCQDVAATPVARYPPIVASMTADSKAARLRRIERWQATVHAAESVDEKLRILTKMQFMKYMVYPQTFALNADRWYQYFTKTVFLSGLPPPPAEPEPEPEPEPEPALDLAALRAVACDCLLQEHFYLRRRRRVHRYEESEVISLPFLDQLVSTLVGLLSPHNPALAAAALDYRCPVHFYWVRGEEIIPRGHRRGRIDDLRYQIDDKPNNQIRISKQLAEFVPLDYSVPIEIPTIKCKPDKLPLFKRQYENHIFVGSKTADPCCYGHTQFHLLPDKLRRERLLRQNCADQIEVVFRANAIASLFAWTGAQAMYQGFWSEADVTRPFVSQAVITDGKYFSFFCYQLNTLALTTQADQNNPRKNICWGTQSKPLYETIEDNDVKGFNDDVLLQIVHFLLNRPKEEKSQLLEN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
92
Xenopus
67
Zebrafish
50
Chicken
50
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NP92 As Substrate

Site PTM Type Enzyme
Y44 Phosphorylation
K79 Ubiquitination
T97 Phosphorylation
R230 Methylation
K236 Ubiquitination
K245 Ubiquitination
Y255 Phosphorylation
K275 Methylation
K287 Ubiquitination
K399 Ubiquitination
K433 Ubiquitination

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Heart, skeletal muscle, kidney and liver. Lower expression in placenta and peripheral blood leukocytes.

Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. mL65 forms a heterodimer with mL37.

Family&Domains:

Belongs to the mitochondrion-specific ribosomal protein mL65 family.

Restrictive clause

 

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For Research Use Only.
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