MPHOSPH9 Antibody - #DF4184

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Product: MPHOSPH9 Antibody
Catalog: DF4184
Description: Rabbit polyclonal antibody to MPHOSPH9
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 78 KD; 133kD(Calculated).
Uniprot: Q99550
RRID: AB_2836549

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(88%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MPHOSPH9 Antibody detects endogenous levels of total MPHOSPH9.
RRID:
AB_2836549
Cite Format: Affinity Biosciences Cat# DF4184, RRID:AB_2836549.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

M phase phosphoprotein 9; M-phase phosphoprotein 9; Mphosph9; MPP 9; MPP9; MPP9_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q99550(MPP9_HUMAN) >>Visit HPA database.

Gene(ID):
MPHOSPH9(10198)
Sequence:
MEEFDLVKTLHKTSSSVGSDENSLHSLGLNLNTDRSSPHLSTNGVSSFSGKTRPSVIQGTVEVLTSLMQELQNSGKTDSELWKNCETRWLQLFNLVEKQCQEQIVAQQEQFHNQIQHIQEEIKNLVKLQTSSASLASCEGNSSNKQVSSESQMGFFSLSSERNESVIHYPESTEPEIQQEMSTSQPDCNVDSCSVSSGYGTFCISELNLYKSKDPKEFMEHIDVPKGQYVAPAVPAESLVDGVKNENFYIQTPEECHVSLKEDVSISPGEFEHNFLGENKVSEVYSGKTNSNAITSWAQKLKQNQPKRAHVEDGGSRSKQGNEQSKKTPIEKSDFAAATHPRAFYLSKPDETPNAWMSDSGTGLTYWKLEEKDMHHSLPETLEKTFISLSSTDVSPNQSNTSNEMKLPSLKDIYYKKQRENKQLPERNLTSASNPNHPPEVLTLDPTLHMKPKQQISGIQPHGLPNALDDRISFSPDSVLEPSMSSPSDIDSFSQASNVTSQLPGFPKYPSHTKASPVDSWKNQTFQNESRTSSTFPSVYTITSNDISVNTVDEENTVMVASASVSQSQLPGTANSVPECISLTSLEDPVILSKIRQNLKEKHARHIADLRAYYESEINSLKQKLEAKEISGVEDWKITNQILVDRCGQLDSALHEATSRVRTLENKNNLLEIEVNDLRERFSAASSASKILQERIEEMRTSSKEKDNTIIRLKSRLQDLEEAFENAYKLSDDKEAQLKQENKMFQDLLGEYESLGKEHRRVKDALNTTENKLLDAYTQISDLKRMISKLEAQVKQVEHENMLSLRHNSRIHVRPSRANTLATSDVSRRKWLIPGAEYSIFTGQPLDTQDSNVDNQLEETCSLGHRSPLEKDSSPGSSSTSLLIKKQRETSDTPIMRALKELDEGKIFKNWGTQTEKEDTSNINPRQTETSVNASRSPEKCAQQRQKRLNSASQRSSSLPPSNRKSSTPTKREIMLTPVTVAYSPKRSPKENLSPGFSHLLSKNESSPIRFDILLDDLDTVPVSTLQRTNPRKQLQFLPLDDSEEKTYSEKATDNHVNHSSCPEPVPNGVKKVSVRTAWEKNKSVSYEQCKPVSVTPQGNDFEYTAKIRTLAETERFFDELTKEKDQIEAALSRMPSPGGRITLQTRLNQEALEDRLERINRELGSVRMTLKKFHVLRTSANL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
88
Xenopus
71
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99550 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
K12 Ubiquitination
K83 Ubiquitination
K127 Ubiquitination
K216 Ubiquitination
K244 Ubiquitination
T252 Phosphorylation
S267 Phosphorylation
K280 Ubiquitination
K288 Ubiquitination
K300 Ubiquitination
T328 Phosphorylation
K332 Ubiquitination
S333 Phosphorylation
K372 Ubiquitination
S377 Phosphorylation
T392 Phosphorylation
K453 Ubiquitination
K514 Ubiquitination
S516 Phosphorylation
K522 Ubiquitination
K628 Ubiquitination
K637 Ubiquitination
K667 Ubiquitination
K690 Ubiquitination
K729 Acetylation
K729 Ubiquitination
K739 Acetylation
K743 Acetylation
K789 Ubiquitination
T820 Phosphorylation
Y838 Phosphorylation
S839 Phosphorylation
T842 Phosphorylation
S867 Phosphorylation
S873 Phosphorylation
S874 Phosphorylation
K886 Methylation
K906 Ubiquitination
K909 Ubiquitination
K917 Ubiquitination
S937 Phosphorylation
S958 Phosphorylation
T977 Phosphorylation
S984 Phosphorylation
S988 Phosphorylation
S994 Phosphorylation
K1033 Ubiquitination
K1046 Ubiquitination
K1091 Ubiquitination
T1096 Phosphorylation
K1107 Ubiquitination
K1123 Ubiquitination
K1125 Ubiquitination
S1137 Phosphorylation
S1166 Phosphorylation
K1172 Acetylation

Research Backgrounds

PTMs:

Phosphorylated in M (mitotic) phase.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Golgi apparatus membrane>Peripheral membrane protein.
Note: Localizes to the distal and proximal end of centriole pairs in duplicated centrosomes. In ciliated cells, localizes to the distal and proximal end of daughter centriole and proximal of the mother centriole but not in the distal end of the mother centriole.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

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