N4BP1 Antibody - #DF4222

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Product: N4BP1 Antibody
Catalog: DF4222
Description: Rabbit polyclonal antibody to N4BP1
Application: WB IF/ICC
Reactivity: Human
Prediction: Mouse, Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 100 KD; 100kD(Calculated).
Uniprot: O75113
RRID: AB_2836573

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Mouse(%), Pig(100%), Bovine(82%), Horse(91%), Sheep(91%), Rabbit(90%), Dog(91%)
Clonality:
Polyclonal
Specificity:
N4BP1 Antibody detects endogenous levels of total N4BP1.
RRID:
AB_2836573
Cite Format: Affinity Biosciences Cat# DF4222, RRID:AB_2836573.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ31821; KIAA0615; MGC176730; N4BP1; N4BP1_HUMAN; NEDD4 binding protein 1; NEDD4-binding protein 1;

Immunogens

Immunogen:
Uniprot:

O75113(N4BP1_HUMAN) >>Visit HPA database.

Gene(ID):
N4BP1(9683)
Expression:
O75113 N4BP1_HUMAN:

Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.

Sequence:
MAARAVLDEFTAPAEKAELLEQSRGRIEGLFGVSLAVLGALGAEEPLPARIWLQLCGAQEAVHSAKEYIKGICEPELEERECYPKDMHCIFVGAESLFLKSLIQDTCADLCILDIGLLGIRGSAEAVVMARSHIQQFVKLFENKENLPSSQKESEVKREFKQFVEAHADNYTMDLLILPTSLKKELLTLTQGEENLFETGDDEVIEMRDSQQTEFTQNAATGLNISRDETVLQEEARNKAGTPVSELTKQMDTVLSSSPDVLFDPINGLTPDEEALSNERICQKRRFSDSEERHTKKQFSLENVQEGEILHDAKTLAGNVIADLSDSSADSENLSPDIKETTEEMEYNILVNFFKTMGYSQEIVEKVIKVYGPSTEPLLLLEEIEKENKRFQEDREFSAGTVYPETNKTKNKGVYSSTNELTTDSTPKKTQAHTQQNMVEKFSQLPFKVEAKPCTSNCRINTFRTVPIEQKHEVWGSNQNYICNTDPETDGLSPSVASPSPKEVNFVSRGASSHQPRVPLFPENGLHQQPEPLLPNNMKSACEKRLGCCSSPHSKPNCSTLSPPMPLPQLLPSVTDARSAGPSDHIDSSVTGVQRFRDTLKIPYKLELKNEPGRTDLKHIVIDGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITVFVPQWRTRRDPNVTEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLLHLADKTGGIIVTNDNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDPLGRSGPRLEEFLQKEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSHQPPTRIQGAPSSHWLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELREALLKIFPDSEQRLKIDQILVAHPYMKDLNALSAMVLD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
91
Sheep
91
Dog
91
Rabbit
90
Bovine
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75113 As Substrate

Site PTM Type Enzyme
K16 Ubiquitination
K70 Ubiquitination
K144 Ubiquitination
S150 Phosphorylation
S226 Phosphorylation
K239 Ubiquitination
T242 Phosphorylation
S245 Phosphorylation
T248 Phosphorylation
T253 Phosphorylation
S256 Phosphorylation
S258 Phosphorylation
T270 Phosphorylation
S288 Phosphorylation
S290 Phosphorylation
K297 Ubiquitination
S300 Phosphorylation
S335 Phosphorylation
K366 Ubiquitination
K369 Ubiquitination
K386 Ubiquitination
S398 Phosphorylation
T401 Phosphorylation
T406 Phosphorylation
K408 Ubiquitination
K412 Ubiquitination
Y415 Phosphorylation
S416 Phosphorylation
S417 Phosphorylation
T418 Phosphorylation
S425 Phosphorylation
T426 Phosphorylation
K428 Ubiquitination
K429 Ubiquitination
K441 Ubiquitination
K448 Sumoylation
K448 Ubiquitination
K452 Ubiquitination
K471 Ubiquitination
T489 Phosphorylation
S493 Phosphorylation
S495 Phosphorylation
S498 Phosphorylation
S500 Phosphorylation
K502 Ubiquitination
S508 Phosphorylation
R509 Methylation
R517 Methylation
K539 Ubiquitination
S554 Phosphorylation
S559 Phosphorylation
T560 Phosphorylation
S562 Phosphorylation
S579 Phosphorylation
K601 Ubiquitination
K605 Ubiquitination
K609 Ubiquitination
K618 Ubiquitination
K634 Ubiquitination
S734 Phosphorylation
K773 Ubiquitination
S838 Phosphorylation
K863 Ubiquitination

Research Backgrounds

Function:

Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity).

PTMs:

Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated with SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies (By similarity).

Subcellular Location:

Nucleus>Nucleolus. Nucleus>PML body.
Note: Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.

Subunit Structure:

Interacts with NEDD4. Interacts with ITCH (via WW domain 2) (By similarity).

Family&Domains:

Belongs to the N4BP1 family.

Restrictive clause

 

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